You searched for: Enzymes

Supplier: MP Biomedicals

Aspergillus niger Type X glucose oxidase

Supplier: MP Biomedicals

Soluble in distilled water or dilute buffer; dissolves readily at 5 mg/ml in 0,1 M potassium phosphate pH 7,4.

Supplier: MP Biomedicals

Fig tree Ficin

Supplier: Thermo Fisher Scientific

CAS No.: 9001-73-4

Supplier: Abnova

Recombinant Poly(A) Polymerase (from E. coli)

Supplier: MP Biomedicals

Myrothecium sp. Bilirubin oxidase

Supplier: Thermo Fisher Scientific

Contains either Lactose or Maltodextrin excipient

Supplier: Abnova

Recombinant mRNA Cap 2'-O-methyltransferase (from E. coli)

Supplier: Abnova

Synthetic Yeast Inorganic Pyrophosphatase (from E. coli)

Supplier: MP Biomedicals

One unit will oxidise 1,0 µmole of formaldehyde to formic acid per minute at pH 7,5 and 37 °C.

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,01 M Sodium Phosphate pH 7,5 to give a clear colourless solution.

Supplier: MP Biomedicals

Sorbitol dehydrogenase has been used in a study to investigate osmotic stress induced oxidative damage as a possible mechanism of cataract formation in diabetes.

Supplier: MP Biomedicals

Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.

Supplier: Cytiva

Exonuclease I acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction, producing 5'-mononucleotides. Applications include eliminating residual single-stranded DNA containing a 3'-terminus, measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA, measuring DNA helicase activity.

Supplier: MP Biomedicals

One unit decomposes 1,0 µmole of hydrogen peroxide per minute at pH 7,0, 25 °C.

Supplier: MP Biomedicals

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7,0. Prepare solutions immediately before use.

Supplier: MP Biomedicals

Activity: 10 units/mg solid or more (containing approx. 20% of stabilisers). Unit definition: One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8,0 at 37 °C. Ref.: P.J.G. Mann and J.H. Quastel, Biochem. J., 31: 869 (1937)

Supplier: MP Biomedicals

One unit will hydrolyse 1,0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4,5 and 30 °C.

Supplier: MP Biomedicals

One unit catalyses the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6,9 at 20 °C).

Supplier: MP Biomedicals

Trypsin 1:250 from pig pancreas is used to release adherent cells from tissue culture plates for passaging. It was used in the isolation and culture of human endothelial cells.

Supplier: MP Biomedicals

Collagenase is a protease which cleaves the triple-helical protein called collagen. It has extensive use in biological studies, where it is used to prepare isolated cell suspensions.

Supplier: MP Biomedicals

Preparation Method
Produced from 3× crystallised chymotrypsinogen

α-Chymotrypsin is used for treating pancreatic insufficiency and in traumatology.

Chymotrypsin preferentially catalyses the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine, and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. In addition to bonds involving aromatic amino acids, chymotrypsin catalyses at a high rate the hydrolysis of bonds of leucyl, methionyl, asparaginyl, and glutamyl residues. a-Chymotrypsin is a protein consisting of 241 amino acid residues. The molecule has three peptide chains: an A chain of 13 residues, a B chain of 131 residues, and a C chain of 97 residues.

Supplier: MP Biomedicals

Plasminogen is a single-chain glycoprotein found in human plasma and extracellular fluid. Certain activators, such as tissue plasminogen activator (tPA), convert plasminogen to its active form, plasmin. Plasminogen has been used to study its conversion into plasmin.

Supplier: MP Biomedicals

Trypsin consists of a single chain polypeptide of 223 amino acid residues. It is a member of the serine protease family. It composed of two subunits, α-trypsin and β-trypsin. α-Trypsin is composed of two peptide chains and β-trypsin is composed of one chain.

Supplier: MP Biomedicals

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolised in the tricarboxylic acid cycle to produce carbon dioxide, water and energy.

Supplier: MP Biomedicals

Pig Diaphorase (from Heart)

Supplier: Abnova

Recombinant Bovine ENTEROKINASE (from P. pastoris), His-tag

Supplier: MP Biomedicals

Readily soluble in water.

Supplier: MP Biomedicals

Proline specific endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues.

Supplier: MP Biomedicals

Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), Ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) +D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal stability: below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).