You searched for: Enzymes
Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Bovine alpha-Chymotrypsin (from Pancreas)
Supplier: Merck
Bovine alpha-Chymotrypsin (from Pancreas)
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Human lysozyme (Muramidase) (from Milk)
Supplier: Merck
Human lysozyme (Muramidase) (from Milk)
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Lyticase (from Arthrobacter luteus)
Supplier: Merck
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
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Bovine catalase (from Liver)
Supplier: Merck
Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. Used to protect against oxidative damage to proteins, lipids, and nucleic acids.
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Aldehyde dehydrogenase (from Yeast)
Supplier: Merck
Aldehyde dehydrogenase from baker′s yeast catalyses the reduction of pyridine nucleotides by several aldehydes. It catalyses the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. The enzyme has been used to study the production of ethanol and isobutanol.
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Pineapple Bromelain (from Stem)
Supplier: Merck
Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulphate. It has also been used as a Azocasein substrate for the determination of proteolytic activity.
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Chicken lysozyme (Muramidase) (from egg white)
Supplier: Merck
Chicken lysozyme (Muramidase) (from egg white)
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Bovine desoxyribonuclease II Typ V (from Spleen)
Supplier: Merck
Bovine desoxyribonuclease II Typ V (from Spleen)
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Rabbit Triosephosphate isomerase (from Muscle)
Supplier: Merck
Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet.
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alpha-Amylase (from Bacillus licheniformis)
Supplier: Merck
α-Amylase solution is used in total dietary fiber assay.
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Bovine L-lactate dehydrogenase Typ III (from Heart)
Supplier: Merck
Bovine L-lactate dehydrogenase Typ III (from Heart)
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Bovine Ribonuclease B (from Pancreas)
Supplier: Merck
Ribonuclease B from bovine pancreas, purified by affinity chromatography is used in the digestion of RNA during cell cycle platform analysis.
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alpha-Chymotrypsin
Supplier: Merck
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8,75. It selectively hydrolyses peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
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Alcohol oxidase
Supplier: Merck
Alcohol oxidase is from Pichia pastoris used to study protein translocation into peroxisomes. Also used for the bacterial expression and immunological verification of Hv-p68 cDNA clones.
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alpha-Chymotrypsin
Supplier: Merck
Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25,7 kDa and is widely used in pharmaceutical industry.It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein.The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.
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Trichoderma reesei cellulase
Supplier: Merck
Cellulase from Trichoderma reesei is produced by submerged fermentation of a selected strain of the fungus Trichoderma reesei and catalyzes the breakdown of cellulose into glucose, cellobiose, and higher glucose polymers. It corresponds to a molecular mass of 68 kDa with optimum pH and temperature of 6 and 52 °C, respectively.
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Streptomyces griseus chitosanase (from Streptomyces griseus)
Supplier: Merck
Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase.
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Laccase (from Trametes versicolor)
Supplier: Merck
Laccase catalyses the oxidation of phenol containing compounds, including lignin, through the reduction of oxygen to water. The presence of mediators will allow the oxidation of non-phenlic compounds as well. The primary function of laccase is to degrade lignin in fungi.
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Chicken lysozyme (Muramidase) (from egg white)
Supplier: Merck
Lysosyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionisation-time of flight) mass analysis.Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
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Chicken lysozyme (Muramidase) (from egg white)
Supplier: Merck
Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
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Chicken lysozyme (Muramidase) (from egg white)
Supplier: Merck
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6,0 to 9,0). At pH 6,2, maximal activity is observed over a wider range of ionic strengths (0,02 to 0,100 M) than at pH 9,2 (0,01 to 0,06 M).
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Trichoderma reesei cellulase
Supplier: Merck
Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose; to study the biodegradability of bioabsorbable bacterial cellulose (BBC) material. Cellulase from Trichoderma reesei has been used in a study to assess the differential regulation of the cellulase transcription factors XYR1, ACE2, and ACE. Cellulase from Trichoderma reesei has also been used in a study to investigate the liquefaction of hydrothermally pretreated wheat straw at high-solids content.
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Pineapple Bromelain (from Stem)
Supplier: Merck
Bromelain from pineapple stem has been used to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants, as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay, to study its potential analgesic effect and to evaluate its hydrolysing effects on proenkephalin (PENK)-derived peptides containing enkephalin sequences.
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Trichoderma endo-1,3-beta-D-glucanase (from Trichoderma sp.)
Supplier: MEGAZYME
endo-1,3-β-D-Glucanase is isolated from Trichoderma species. These are used in carbohydrate and biofuels research, biochemical enzyme assays and in vitro diagnostic analysis.
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Fructanase
Supplier: MEGAZYME
This purified fructanase mixture is used for the determination of fructn (liquid), in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Linamarase (beta-D-Glucosidase)
Supplier: MEGAZYME
Linamarase (beta-D-Glucosidase)