Anti-BIG1/ARFGEF1 Rabbit Polyclonal Antibody

BOSSBS-12866R

Anti-BIG1/ARFGEF1 Rabbit Polyclonal Antibody

BOSSBS-12866R

Antibody type:
Primary
Antigen name:
ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Antigen symbol:
BIG1/ARFGEF1
Clonality:
Polyclonal
Conjugation:
Unconjugated
Host:
Rabbit
ImmunoChemistry:
Yes
Isotype:
IgG
Reactivity:
Human,
Rat,
Mouse
Western blot:
Yes
Form:
liquid
Gene ID:
10565
Antigen synonyms:
ARFGEP1|p200 ARF GEP1|BIG1_HUMAN.|p200 ARF guanine nucleotide exchange factor|ADPribosylation factor guanine nucleotide exchange factor 1brefeldin A inhibited|ARFGEF1|Brefeldin A inhibited guanine nucleotide exchange protein 1|Brefeldin A inhibited GEP 1
Storage buffer:
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0,09% sodium azide. Store at -20°C for 12 months.
Concentration:
1 ug/ul
Shipping temperature:
4 °C
Purification:
Purified by Protein A.
Size:
100 μg
Pk:
100 µl

Guanine nucleotide-exchange proteins (GEPs) accelerate replacement of bound GDP with GTP and thereby activate ADP-ribosylation factors (ARFs), a family of guanine nucleotide-binding proteins that play an important role in intracellular vesicular trafficking. GEPs comprise two major families, large GEPs that are inhibited by brefeldin A (BFA), a protein that effects Golgi structure and a group of smaller GEPs that are insenstive to BFA. Two genes for GEPs found on human chromosomes 8 and 20 encode BFA sensitive GEPs designated BIG1 and BIG2. Both GEPS contain a sec7 domain that is responsible for their brefeldin inhibition and also their catalytic activity. In vivo, BIG1 and BIG2 exist in macromolecular complexes that move between the Golgi membranes and cytosol. BIG2 associates with PKA regulatory subunits, implying that BIG2 may act as an A kinase-anchoring protein (AKAP) that could coordinate the cAMP and ARF regulatory pathways.

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Type: Primary
Antigen: BIG1/ARFGEF1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat