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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

SDS-Page running gel buffers, 10X concentrate

SDS-Page running gel buffers, 10X concentrate

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

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Tris EDTA buffer, for molecular biology, DNAse, RNAse, protease free ready to use, pH 8,0
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VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

Supplier: VWR Chemicals

The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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In situ hybridisation wash reagent

In situ hybridisation wash reagent

Supplier: ENZO LIFE SCIENCES

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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Buffers, sodium phosphate buffer solutions (0,5 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 7,0

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

J.T.Baker® Cell Lysis Solution, Ultrapure Bioreagent

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

    
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Protein blocking agent, BLOT-QuickBlocker™

Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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TES buffer, 1X, ready to use, pH 7,8

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.

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Depyrogenated Dilution Tubes

Depyrogenated Dilution Tubes

Supplier: FUJIFILM WAKO CHEMICALS

These depyrogenated dilution tubes are made of high-quality borosilicate glass depyrogenated at 250 °C.

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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

    
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Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD

Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Maximum Recovery Diluent is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Gel-clot Reaction Tubes

Gel-clot Reaction Tubes

Supplier: FUJIFILM WAKO CHEMICALS

These Gel-clot Reaction Tubes are made of high-quality borosilicate glass, depyrogenated at 250 °C. This results in an endotoxin-free tube that is designed to perform successful LAL gel clot assays with heat blocks and water baths.

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Buffer, TE Solution (TRIS-EDTA buffer) pH 8,0, Low EDTA for Molecular Biology

Supplier: PanReac AppliChem

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: OMEGA BIO-TEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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North2South™ Hybridisation Buffer

North2South™ Hybridisation Buffer

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

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Tris-Glycine native gel running buffer

Supplier: G-Biosciences

Running Buffer, Tris-Glycine native gel running buffer

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Wash buffer, Pierce™

Wash buffer, Pierce™

Supplier: Thermo Fisher Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

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Equilibration buffer for IPG strips

Supplier: G-Biosciences

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer.

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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)

Pierce™ Lane Marker Non-reducing Sample Buffer (5X)

Supplier: Thermo Fisher Scientific

Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

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DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK

CP buffer, Omega Bio-Tek

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TAE buffer (20X), Pierce™

TAE buffer (20X), Pierce™

Supplier: Thermo Fisher Scientific

20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.

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Electrophoresis running buffers, Pierce™

Electrophoresis running buffers, Pierce™

Supplier: Thermo Fisher Scientific

These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.

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Pierce™ BupH™ Wash Buffers, Dry Blend Pack

Pierce™ BupH™ Wash Buffers, Dry Blend Pack

Supplier: Thermo Fisher Scientific

BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.

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Buffer, alkali solution (3,0 N)

Buffer, alkali solution (3,0 N)

Supplier: Hach

These buffers are used in the determination of calcium and magnesium in brine.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Substrate buffers, Pierce™

Substrate buffers, Pierce™

Supplier: Thermo Fisher Scientific

These stable peroxide and diethanolamine substrate buffers produce optimised, reliable and convenient stock solutions for HRP and alkaline phosphatase assays detection respectively.

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