Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®
Supplier: Merck
Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)
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Buffer, TRIS-tricine-SDS solution 10X concentrate, Sigma-Aldrich®
Supplier: Merck
Running Buffer, TRIS-tricine-SDS buffer solution 10X concentrate
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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®
Supplier: Merck
1M Buffer, also for use in ion-exchange chromatography and electrophoresis.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®
Supplier: Merck
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TRIS solution pH 8,0 (2 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 8,0 (2 mol/l)
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Buffer, TRIS solution pH 8,0 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 8,0 (1 mol/l)
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Buffer, imidazole buffer solution (1 mol/L) BioUltra, Sigma-Aldrich®
Supplier: Merck
Imidazole buffer solution (1 mol/l)
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Buffer, TRIS solution pH 7,4 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)
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Buffer, TRIS solution pH 7,8 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,8 (1 mol/l)
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Buffer, TRIS solution pH 8,0 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 8,0 (1 mol/l)
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Buffer, TRIS solution pH 7,2 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,2 (1 mol/l)
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Borate buffer for HPLC, LiChropur™, Supelco®
Supplier: Merck
Borate buffer, 0.127 - 0.140 M
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Buffer, HEPES solution (1 mol/L), Sigma-Aldrich®
Supplier: Merck
HEPES buffer solution (1 mol/l)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®
Supplier: Merck
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®
Supplier: Merck
Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)
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Buffer, HEPES solution, 1 M, suitable for cell culture, 0,2 μm filtered, BioPerformance Certified, Sigma-Aldrich®
Supplier: Merck
HEPES buffer solution (1 mol/l)
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Buffer, sodium acetate buffer solution pH 7 (3 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)
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Phosphate buffer solution, BioUltra, Supelco®
Supplier: Merck
Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2
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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®
Supplier: Merck
Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)
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Buffer capsules, coloured coded
Supplier: REAGECON
pH buffer capsules, reference standard, pH value: 10,00, ±0,02, Reference temperature: 25 °C, Blue
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4
Supplier: Merck
Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.
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Buffer, TRIS solution pH 9,0 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 9,0 (1 mol/l)
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Dulbecco’s phosphate buffered saline (DPBS), Sigma-Aldrich®
Supplier: Merck
Dulbecco’s phosphate buffered saline is modified with/without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture.
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Buffer, TRIS solution pH 7,5 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®
Supplier: Merck
Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.
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Buffer, Trizma® hydrochloride solution, TRIS solution pH 7,4 (1 mol/L), BioPerformance Certified, Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)
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Phosphate buffered saline (PBS), Sigma-Aldrich®
Supplier: Merck
Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®
Supplier: Merck
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Glycine buffer, Sigma-Aldrich®
Supplier: Merck
For use in the enzymatic determination of lactate using lactic dehydrogenase.