You searched for: Cell Culture Supplements and Additives
The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.
Phenol red, sodium salt
Supplier: Biowest
Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.
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M9 Minimal Salts (Maniatis Formulation), Powder, MP Biomedicals
Supplier: MP Biomedicals
M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.
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Protease inhibitor cocktail, FOCUS™ ProteaseArrest™
Supplier: G-Biosciences
A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.
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Ion channel ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® ion channel ligand library contains 71 ion channel blockers and openers for use in characterising and identifying ion channels in individual cells or tissue.
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Cryopreservation solutions, DMSO-free, pZerve™
Supplier: PROTIDE PHARMACEUTICALS
A unique cryopreservation solution that does not contain DMSO, foetal bovine serum, or animal protein. pZerve™ is ready to use and does not require any dilution or further processing.
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Protease inhibitor cocktail, Mammalian ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.
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Protease inhibitor cocktail, Plant ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.
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Cryopreservation solution, DMSO-free, Cellvation™
Supplier: PROTIDE PHARMACEUTICALS
A unique cryopreservation solution that does not contain DMSO or foetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum-containing medium.
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Supplement Kit for Skeletal Muscle Cell Growth Medium, FCS
Supplier: PROVITRO
The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
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MEM vitamins 100X concentrate
Supplier: Biowest
Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.
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Supplement Kit for MVEC Growth Medium, Advanced, FCS
Supplier: PROVITRO
The supplement kit for microvascular endothelial cell growth medium, advanced is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
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MEM non essential amino acids 100X
Supplier: Biowest
Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.
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Cryopreservation solutions, defined, 2-8CELLsius + DMSO
Supplier: PROTIDE PHARMACEUTICALS
When combined with DMSO, 2-8CELLsius™ can be utilised as a cryopreservation solution. 2-8CELLsius™ + DMSO is a cytoprotective, protein-free solution, with bio-stabilisers that support high post thaw cell viability. Optimal recovery is achieved through a novel approach to cell physiology at ultra-low temperatures.
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TCH defined serum-free growth medium
Supplier: PROTIDE PHARMACEUTICALS
Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.
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TCM defined serum-free growth medium
Supplier: PROTIDE PHARMACEUTICALS
General serum replacement for many cell types from a variety of species. TCM™ is a defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. TCM™ contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.
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Egg yolk tellurite emulsion
Supplier: THERMO FISHER DIAGNOSTICS
Egg yolk tellurite emulsion
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HyClone Cell Boost™ 6 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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Protease inhibitor set
Supplier: G-Biosciences
Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.
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L-alanyl L-glutamine, SG-200, HyClone™
Supplier: HyClone products (Cytiva)
SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.
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Glutamine
Supplier: Biowest
L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.
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Protease inhibitor cocktail, Recom ProteaseArrest™
Supplier: G-Biosciences
Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.
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TM-235 defined serum-free growth medium
Supplier: PROTIDE PHARMACEUTICALS
Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.
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Short-term storage solutions, 2-8CELLsius
Supplier: PROTIDE PHARMACEUTICALS
Ready to use, balanced, cytoprotective, protein-free solution with bio-stabilisers for short-term storage, regenerative medicine, stem cell processing and transportation of biological material. Short-term storage can be achieved at 2 to 8 °C or at ambient temperature for 24 to 48 hours. Does not contain any growth factors, hormones, or antibiotics. 2-8CELLsius™ can also be utilised as a cell washing solution.
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Dispase II solution in PBS, Provitro
Supplier: PROVITRO
Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.
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Protease inhibitor cocktails, ProteCEASE™
Supplier: G-Biosciences
ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.
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Histaminergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® histaminergic and melatonin ligands plate contains 41 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The Histaminergic and Melatonin Ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.
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Hepatotoxicity library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® hepatotoxicity library is a focused collection of 238 compounds with defined and diverse hepatotoxicity, including steatosis, mitochondrial toxicity, Mallory body formation, cholestatic effects, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.
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Trypsin
Supplier: Biowest
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.
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Metabotropic glutamatergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® metabotropic glutamatergic ligands plate contains 49 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The metabotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.
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GABAergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The GABAergic igands library contains 56 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein-coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The GABAergics ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 CNS receptor ligands.