"protein purification system"

Supplier: G-Biosciences

The Pearl™ IgG purification resin allows for the one-step purification of immunoglobulin G from serum. The resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: OMNIFIT

EZ columns are designed to suit the majority of standard laboratory chromatography applications. They are ideal for aqueous systems and compatible with solvents used in common liquid chromatography applications such as protein purification.

Supplier: Thermo Fisher Scientific

The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: Thermo Fisher Scientific

The Thermo Scientific KingFisher Apex represents the pinnacle of automated purification for DNA, RNA, proteins, and cells. Building on decades of KingFisher product value and excellence, we’ve combined superior instrument capabilities with complete touch screen-based control to deliver unrivalled flexibility and performance. The KingFisher Apex makes sample prep simple.

Supplier: Abcam

His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.

Supplier: Rockland Immunochemicals

Protein G is a surface protein of two groups of streptococcal bacteria that has ability to bind immunoglobulins and also to albumins. Protein G are prepared from chromatographically pure protein G.

Supplier: G-Biosciences

Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants.

Supplier: G-Biosciences

SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.

Supplier: Abcam

GTP-Agarose (ab270533) comprises GTP attached to agarose beads.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Supplier: Biotium

Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

Supplier: Abcam

Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG® tag sequence) (ab270704) is an affinity chromatography medium designed for easy, one-step purification or immunoprecipitation of DYKDDDDK (flag) at N-terminal, C-terminal and internal locations of a fusion protein.

Supplier: Genscript

Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.