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683 výsledků pro "buffers"

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Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Supplier: Rockland Immunochemicals

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Buffered NaCl Peptone Solution pH 7,0

Supplier: LIOFILCHEM

Buffered NaCl Peptone Solution pH 7,0 is used for dissolving, suspending and diluting test samples. Its composition conforms to recommendations in the Harmonised Pharmacopoeias for the microbiological examination of non-sterile products.

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RIPA cell lysis buffer 2

Supplier: ENZO LIFE SCIENCES

RIPA cell lysis buffer 2, Enzo Life Sciences

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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Optimizer Buffer™ for optimal cross-linking and modification

Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Cell lysis buffer 4, Enzo Life Sciences

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HEPES Buffer Solution 1 M, HyClone™

HEPES Buffer Solution 1 M, HyClone™

Supplier: HyClone products (Cytiva)

Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

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Non-animal protein blocking Agent, NAP-BLOCKER™

Non-animal protein blocking Agent, NAP-BLOCKER™

Supplier: G-Biosciences

NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.

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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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PBS and TBS buffers

PBS and TBS buffers

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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PBS (endotoxin-free), Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.

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Buffer, PBS tablets

Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

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Mini flip-top vial with 9 ml buffered peptone water broth, BPPFV9BPW

Mini flip-top vial with 9 ml buffered peptone water broth, BPPFV9BPW

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Buffered Peptone Water Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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SSPE Buffer, 20X concentrate, pH 7,4

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

VWR® Buffer, Ammonium Chloride, pH 10, Reag. Ph.Eur.

Supplier: VWR Chemicals

The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Caspase assay buffer

Supplier: ENZO LIFE SCIENCES

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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Extraction reagents

Supplier: ENZO LIFE SCIENCES

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Neutralising reagent

Supplier: ENZO LIFE SCIENCES

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

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HDAC assay buffer II

Supplier: ENZO LIFE SCIENCES

50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA.

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TPE buffer, 10X tris-phosphate-EDTA, pH 8

TPE buffer, 10X tris-phosphate-EDTA, pH 8

Supplier: Rockland Immunochemicals

Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)

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Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solutions for cell culture applications.

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SSC buffer, 20X concentrate, pH 7,0

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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Tween-20 solution (10%)

Supplier: ENZO LIFE SCIENCES

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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