buffers

Pufry odolávají změnám pH a chrání vzorky při přidání kyselin nebo zásad. Práškové a kapalné formy účinně sledují proteiny během elektroforézy, izolace a separace. Slané pufry lze také použít ke kalibraci a standardizaci pH metrů pro přesné měření. Blokovací látky zabraňují navázání protilátek na místa bez membrány, která by mohla vytvořit rušivé pozadí. Pufry mohou být k dispozici v různých koncentracích, aby vyhovovaly jakýmkoli potřebám, a to v kapalné nebo dehydratované formě.

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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Cesium nitrate, ionization buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Cesium Nitrate Buffer solution

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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Blocking buffers, Protein-Free™

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

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Diluent buffer, 10X concentrate, for dab membrane substrate

Supplier: Rockland Immunochemicals

Concentrate diluent buffer for DAB membrane substrate, 10X

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: OMEGA BIO-TEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Supplier: Thermo Fisher Scientific

Pufr pro elektroforézu, Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.