buffers
Pufry odolávají změnám pH a chrání vzorky při přidání kyselin nebo zásad. Práškové a kapalné formy účinně sledují proteiny během elektroforézy, izolace a separace. Slané pufry lze také použít ke kalibraci a standardizaci pH metrů pro přesné měření. Blokovací látky zabraňují navázání protilátek na místa bez membrány, která by mohla vytvořit rušivé pozadí. Pufry mohou být k dispozici v různých koncentracích, aby vyhovovaly jakýmkoli potřebám, a to v kapalné nebo dehydratované formě.
VWR®, SSC buffer, 20X, ultra-pure grade
Supplier: VWR Chemicals
SSC buffer is a hybridisation buffer for Northern and Southern blots.
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MMP-3 fluorescent assay buffer
Supplier: Enzo Life Sciences
MMP-3 Fluorescent assay buffer
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Blocking buffers
Supplier: Rockland Immunochemicals
A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
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Buffer HF-Improved
Supplier: Thermo Fisher Scientific
Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: PanReac AppliChem
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology
Supplier: VWR Chemicals
Ready to use Tris-EDTA buffer.
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MMP-1 colorimetric assay buffer
Supplier: Enzo Life Sciences
MMP-1 colorimetric assay buffer
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Permeabilisation buffer
Supplier: Biotium
Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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TE Buffer (1X) pH 8,0 for Molecular Biology
Supplier: Apollo Scientific
Cloning Buffer, TE buffer (1X)
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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