141 výsledků pro: "TriLink BioTechnologies"

Supplier: TriLink BioTechnologies

Cas9 Nickase mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.

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CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

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Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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The Antibody-Oligonucleotide All-in-One Conjugation kit offers TurboLink™ catalysed conjugation based on Solulink’s proprietary linking technology to prepare antibody-oligonucleotide conjugates without chromatography. The kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

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This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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Fluorescein Antibody Conjugation kit offers an efficient method that delivers >95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labelled fluorescein-antibody conjugates from 100 µg of any user-supplied antibody.

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Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

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TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate

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TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

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Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

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ChromaLink™ Didoxigenin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.

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TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate

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TriLink® 2'-O-Methylcytidine-5'-Triphosphate

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Streptavidin magnetic beads and agarose with biotin-binding capacity as much as 12× higher than comparable products.

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TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

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CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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TriLink® 5-Methyluridine-5'-Triphosphate

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A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.

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2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

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 BspQI (isoschizomers: SapI, PciSI and LguI) is a Type IIS restriction enzyme known for its unique cleavage properties. It recognizes asymmetric DNA sequences, cleaves the double-stranded DNA outside of the recognition site, and generates a 5′ overhang.

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TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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TriLink® N1-Propylpseudouridine-5'-Triphosphate

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N,N-Dimethylformamid, anhydrous

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TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

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TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.