362 výsledků pro: "OMEGA BIO-TEK"

Supplier: OMEGA BIO-TEK

Souprava E-Z 96® Plant DNA Kit umožňuje rychlou a spolehlivou izolaci vysoce kvalitní buněčné DNA ze široké škály rostlinných druhů a tkání v 96-jamkovém formátu. Souprava E-Z 96® Plant DNA Kit využívá systému pufrů převzatých ze systému SP Plant DNA, jenž je vhodný pro širokou škálu rostlin, včetně druhů obsahujících neobvykle vysoké hladiny fenolických substancí nebo polysacharidů (například vzorky bavlníku, borovice nebo podzemnice). V každé jamce je možno do 1 hodiny zpracovat až 50 mg čerstvé tkáně, případně 12 mg suché tkáně. Systém kombinuje schopnost HiBind matrice reverzibilně vázat nukleové kyseliny s rychlostí a univerzálností E-Z 96® DNA destičky, což umožňuje odstranění polysacharidů, fenolických sloučenin a inhibitorů enzymů z lyzátů rostlinných tkání. Purifikovaná DNA je vhodná pro PCR, restrikční štěpení i hybridizační techniky.

Supplier: OMEGA BIO-TEK

Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.

Supplier: OMEGA BIO-TEK

Purify endotoxin free (<0,1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

Supplier: OMEGA BIO-TEK

E-Z® 96 RNA plates (10 x 96), E-Z 96®, Počet jamek: 96

Supplier: OMEGA BIO-TEK

Souprava pro izolaci DNA ze stolice, E.Z.N.A.® Stool DNA Kit, umožňuje rychlou a spolehlivou izolaci vysoce kvalitní celkové DNA z čerstvých i zmražených vzorků stolice. Za méně než 60 minut může být zpracováno až 200 mg vzorků stolice. Při eliminaci huminových kyselin, polysacharidů, sloučenin fenolu a inhibitorů enzymů z vzorků stolice systém kombinuje schopnost HiBind matrixu vázat nukleovou kyselinu a rychlost a všestrannost spin kolonek. Purifikovaná DNA je vhodná pro PCR, restrikční štěpení a techniky na bázi hybridizace.

Supplier: OMEGA BIO-TEK

High-throughput DNA and RNA isolation from cultured cells and tissue using magnetic beads.

Supplier: OMEGA BIO-TEK

Souprava pro izolaci bakteriální DNA Mag-Bind® umožňuje rychlou a spolehlivou izolaci genomové DNA (gDNA) vysoké kvality z široké škály druhů bakterií. Naráz může být zpracováváno až 0,5 ml gram pozitivních či gram negativních bakteriálních kultur. Klíčem k tomuto systému jsou částice Mag-Bind®, které v reversibilním procesu naváží za jistých optimálních podmínek DNA či RNA a umožní tak odstranění proteinů či jiných kontaminantů.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum and fecal samples.

Supplier: OMEGA BIO-TEK

VHB buffer must be diluted with ethanol before use.

Supplier: OMEGA BIO-TEK

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

Nucleic Acid Purification Kits and Reagents, cHTR reagent, 50 ml

Supplier: OMEGA BIO-TEK

The Mag-Bind® Blood and Tissue DNA HDQ 96 Kit is designed for rapid and reliable isolation of high quality genomic DNA from 100 to 200 μl blood samples, buccal swabs, buffy coat and cultured cells. All heating steps that limit robotic applications have been removed to allow for faster processing. Mag-Bind® particles HDQ provide quick magnetic response time reducing overall processing time. Utilising paramagnetic particles provides high quality DNA that is suitable for direct use in most downstream applications, such as qPCR, PCR and Next Generation Sequencing. The Mag-Bind® HDQ Blood DNA and Tissue 96 Kit is compatible with many robotic liquid handlers and magnetic processors, including Thermo Scientific KingFisher® Flex.

Supplier: OMEGA BIO-TEK

This dNTP set with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.

Supplier: OMEGA BIO-TEK

The E-Z 384 Plant DNA HT Kit enables rapid and high–throughput isolation of genomic DNA from a wide variety of plant tissue samples. Up to 768 fresh, frozen, or dried plant tissue samples rich in polyphenols, polysaccharides or having a lower DNA content can be processed in approximately 1,5 hours when processing two plates in parallel. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of silica plate technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. The purification procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with a unique binding system to provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. The eluted DNA is inhibitor-free and ready for downstream applications such as genotyping, plant breeding, population level analyses, Next Generation Sequencing, PCR, and more.

Supplier: OMEGA BIO-TEK

Pro enzymatické štěpení během přípravy DNA a RNA. OB Proteáza i Proteináza K nabízejí širokou substrátovou specifitu s vysokou aktivitou pro širokou škálu solí, denaturačních činidel a detergentů, pH a teplotních podmínek. Proteináza K je proteáza subtilisinového typu izolovaná ze saprofytické houby Tritirachiumalbum a je zvláště vhodná pro krátké doby trávení. Má vysokou specifickou aktivitu, která zůstává stabilní v širokém rozmezí teplot a hodnot pH s podstatně zvýšenou aktivitou při vyšších teplotách.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Blood RNA 96 Kit is designed for rapid and reliable isolation of total and viral RNA from mammalian whole blood. The Mag-Bind® bead technology provides high-quality RNA, which is suitable for direct use in most downstream applications, such as amplifications and enzymatic reactions.

Supplier: OMEGA BIO-TEK

E-Z Select® 24-well Plate, 25 ml is designed for performing large volume applications on automated platforms such as Hamilton Microlab® STAR or equivalent.

Supplier: OMEGA BIO-TEK

Isolate DNA from tissues, buccal swabs, cultured cells, whole blood, body fluids, paraffin-embedded tissues, and mouse tail snips using mini spin columns.

Supplier: OMEGA BIO-TEK

Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

Supplier: OMEGA BIO-TEK

Isolate DNA from soil and water samples using magnetic beads.

Supplier: OMEGA BIO-TEK

TL buffer, Omega Bio-Tek

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.

Supplier: OMEGA BIO-TEK

RNA Wash Buffer I

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral RNA from cell free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.

Supplier: OMEGA BIO-TEK

Microplate seal, Adhesive film/foil/tape (96/384 wells), Single sheet/mat/strip, Sealing film, SealPlate®

Supplier: OMEGA BIO-TEK

Selektivní štěpení proteázou uvolňuje z FFPE vzorků mikrogramová množství RNA. Purifikované nukleové kyseliny, ačkoliv vysoce fragmentované, jsou vhodné pro širokou škálu následných genomových analýz a analýz genové exprese. Soupravy E.Z.N.A.® obsahující centrifugační kolonky jsou ideální pro nízký počet vzorků, zatímco Mag-Bind® soupravy fungující na principu magnetických kuliček jsou navrženy speciálně pro uživatele s vysokým počtem vzorků s možností automatizace.