362 výsledků pro: "OMEGA BIO-TEK"

Supplier: OMEGA BIO-TEK

Isolate DNA from soil and water samples using magnetic beads.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

Supplier: OMEGA BIO-TEK

QVL lysis buffer

Supplier: OMEGA BIO-TEK

Microplate seal, Adhesive film/foil/tape (96/384 wells), Single sheet/mat/strip, Sealing film, SealPlate®

Supplier: OMEGA BIO-TEK

The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: OMEGA BIO-TEK

Mag-Bind® Particles RQ

Supplier: OMEGA BIO-TEK

Isolate DNA from up to 10 ml blood using magnetic beads.

Supplier: OMEGA BIO-TEK

This dNTP mix with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabelled dNTPs.

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

Supplier: OMEGA BIO-TEK

Pre-scripted solution for purification of high-quality DNA from blood, saliva, cultured cells, or fresh or frozen tissue.

Supplier: OMEGA BIO-TEK

Accessories for MagBinder® Fit24 Nucleic Acid Purification System

Supplier: OMEGA BIO-TEK

Binding buffer XP2, Omega Bio-Tek

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® Water DNA Kit je navržena pro izolaci vysoce čisté buněčné DNA z vodních vzorků obsahujících huminové kyseliny a inhibitory PCR. Tato souprava je velice účinná při izolaci DNA z bakterií, u kterých se těžko provádi lyze, z hub a z řas, které se nachází ve vodě z vodovodního řádu, jezerech, řekách a v kanalizaci. DNA purifikovaná pomocí soupravy E.Z.N.A.® Water DNA Kit je připravena pro následné aplikace, jako jsou PCR, Southern blotting a anylýza SNP.

Supplier: OMEGA BIO-TEK

Souprava E.Z.N.A.® MicroElute® Genomic DNA Kit nabízí rychlou a snadnou metodu izolace genomové DNA z malých vzorků nebo vzorků o malých objemech pro konzistentní výsledky PCR a analytických testů. Díky reverzibilním vazebným vlastnostem materiálů na bázi oxidu křemičitého, rychlosti technologie centrifugačních minikolonek a specifickým pufrům systém umožňuje purifikaci genomové DNA až do velikosti 60 kb. Vzorky jsou nejprve lyzovány za denaturujících podmínek a pak aplikovány na kolonku HiBind MicroElute® Column, kde dochází k navázání DNA, zatímco zbytky buněk, hemoglobin a další proteiny jsou efektivně vymyty. Vysoce kvalitní DNA je možno z kolonky MicroElute® eluovat do 10 až 15 µl deionizované vody nebo elučního pufru.

Supplier: OMEGA BIO-TEK

Taq DNA polymerase is a thermostable enzyme that can withstand prolonged incubation at temperatures up to 95°C without significant loss of activity.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIO-TEK

Nucleic Acid Purification Kits and Reagents, MicroElute® DNA columns

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: OMEGA BIO-TEK

The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

Supplier: OMEGA BIO-TEK

Selektivní štěpení proteázou uvolňuje z FFPE vzorků mikrogramová množství DNA. Purifikované nukleové kyseliny, ačkoliv vysoce fragmentované, jsou vhodné pro širokou škálu následných genomových analýz a analýz genové exprese. Soupravy E.Z.N.A.® obsahující centrifugační kolonky jsou ideální pro nízký počet vzorků, zatímco Mag-Bind® soupravy fungující na principu magnetických kuliček jsou navrženy speciálně pro uživatele s vysokým počtem vzorků s možností automatizace.

Supplier: OMEGA BIO-TEK

DNA gel loading buffer

Supplier: OMEGA BIO-TEK

The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

Supplier: OMEGA BIO-TEK

Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

Supplier: OMEGA BIO-TEK

Automation-ready, prefilled 96-well plates for quick and convenient DNA extraction from blood, saliva, cultured cells, and swabs for magnetic processors.

Supplier: OMEGA BIO-TEK

The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.

Supplier: OMEGA BIO-TEK

E-Z® 96 DNA plates are silica glass fibre plates can bind up to 50 μg of genomic DNA or 20 μg of plasmid DNA per prep. Each well has a capacity of 1 ml and the plates are semi skirted. E-Z 96 DNA plates can be processed in vacuum manifolds and/or by centrifugation.

Supplier: OMEGA BIO-TEK

1 kbp DNA marker je ideální pro stanovení velikosti dvouřetězcové DNA od 1 kbp do 10 kbp. Marker se skládá z osmi fragmentů, již obsahuje nanášecí pufr a je připraven k použití.