VWR® UltraPure, Tris-glycine buffers, 10X concentrate
Supplier: VWR Chemicals
Running buffers for both 1-D and 2-D protein gel electrophoresis.
Expand 3 Items
VWR® PerfectBlue Maxi ExW 'Revolution', Wide format gel system
Supplier: VWR Collection
The Maxi ExW 'Revolution' offers built-in buffer recirculation - without the need for pumps or additional tubing. The Maxi ExW 'Revolution' offers the same high throughput, rapid separation as the Maxi ExW model but, with the position of the cathode and a rising tube, the system uses hydrogen bubbles to slowly mix the buffer. This system thereby avoids the build-up of pH or ionic gradients for electrophoresis of maximum quality and stability.
Expand 1 Items
VWR®, Accessories for PerfectBlue Vertical Gel Systems
Supplier: VWR Collection
Vertical gel electrophoresis system, accessory, Casting stand, Spare silicone rubber seals for upper buffer chamber/plate holders Cast-It M, Pro: PerfectBlue vertical gels
Expand 4 Items
VWR® Transfer Buffer Concentrate, CAPS 10X
Supplier: VWR Chemicals
For transfer of protein to membranes for sequencing or blotting.
Expand 1 Items
VWR®, MOPS 10X buffer for biotechnology
Supplier: VWR Chemicals
MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.
Expand 1 Items
VWR® A.C.E.™, Sequencing buffers
Supplier: VWR Chemicals
A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.
Expand 1 Items
Glycerin, anhydrous ≥99.5%, Ultrapure Bioreagent for biotechnology, for electrophoresis, J.T.Baker®
Supplier: Avantor
Glycerol, anhydrous, Ultrapure Bioreagent, for enzyme stabilisation, freezing and electrophoresis buffers.
Expand 2 Items
VWR®, Tris-borate-EDTA (TBE) buffer
Supplier: VWR Chemicals
TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).
Expand 9 Items
VWR® PerfectBlue Mini L Revolution, Gel System
Supplier: VWR Collection
The 'Revolution' series includes a buffer circulation system, powered by the electrodes themselves. Hydrogen bubbles produced at the cathode pass up a diagonally positioned pipe connected to the two sides of the tank producing a gentle circulation of the buffer. The result is no pH and ionic gradient – without need of mechanical pumps.
Expand 1 Items
VWR®, SSC buffer, 20X, ultra-pure grade
Supplier: VWR Chemicals
SSC buffer is a hybridisation buffer for Northern and Southern blots.
Expand 1 Items
VWR® Gentle ReView™, Stripping Buffer
Supplier: VWR Chemicals
Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.
Expand 2 Items
VWR® PerfectBlue Maxi M 'Revolution', Gel system
Supplier: VWR Collection
Based on the Maxi M model, the Maxi M 'Revolution' offers a built-in buffer circulation system, powered by the system’s electrodes. At the cathode, hydrogen bubbles are produced and pass up a diagonally positioned pipe connected to the two sides of the tank to produce a gentle and continual circulation of the buffer. The system nulls any pH and ionic gradient – without the fuss of mechanical pumps. The system offers 6 comb positions enabling the gel to be partitioned into 2, 3 or 4 areas. In addition, the system can accommodate any of the 23 standard and microtitre comb designs described for the Maxi M model.
Expand 1 Items
VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer
Supplier: VWR Chemicals
TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.
Expand 3 Items
VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)
Supplier: VWR Chemicals
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
Expand 1 Items
VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
Expand 1 Items
VWR® Buffer, MES-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
Expand 1 Items
VWR®, Red Taq DNA polymerase master mix
Supplier: VWR Chemicals
VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.
Expand 11 Items
VWR® Phosphate Buffered Saline (PBS), Low Phosphate
Supplier: VWR Chemicals
Irradiated solution for use in washing cells.
Expand 1 Items
VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade
Supplier: VWR Chemicals
Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)
Expand 1 Items
2X SDS-PAGE sample buffer without DTT or b-ME
Supplier: Rockland Immunochemicals
Sample buffer for protein electrophoresis.
Expand 1 Items
VWR® Separating Gel Buffer, 4X
Supplier: VWR Chemicals
4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.
Expand 1 Items
VWR® Phosphate Buffered Saline (PBS), High Phosphate
Supplier: VWR Chemicals
Irradiated solution, pH 7,2, for washing blots.
Expand 1 Items
Peqlab PerfectBlue Maxi M 'Revolution', Gel system
Supplier: VWR Peqlab
Based on the Maxi M model, the Maxi M 'Revolution' offers an in-built buffer circulation system, powered by the system's electrodes. At the cathode, hydrogen bubbles are produced and pass up a diagonally positioned pipe connected to the two sides of the tank to produce a gentle and continual circulation of the buffer. The system nulls any pH and ionic gradient – without the fuss of mechanical pumps. The system offers 6 comb positions enabling the gel to be partitioned into 2, 3 or 4 areas. Additionally, the system can accommodate any of the 23 standard and microtitre comb designs described for the Maxi M model.
Expand 1 Items
VWR® UltraPure, PBS and TBS wash buffers
Supplier: VWR Chemicals
Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.
Expand 4 Items
VWR® EZ-Vision®, DNA Ladder, 100 bp
Supplier: VWR Chemicals
EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.
Expand 1 Items
Electrophoresis running buffers
Supplier: G-Biosciences
There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.
Expand 23 Items
VWR®, Loading dye base
Supplier: VWR Chemicals
This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.
Expand 1 Items
VWR® Proteomics Grade, Tris-glycine buffer
Supplier: VWR Chemicals
A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.
Expand 1 Items
VWR®, fyziologický roztok pufrovaný fosfátem (PBS), nízký obsah fosfátů, 10X
Supplier: VWR Chemicals
Ozářený koncentrát PBS, vhodný pro použití při promývání buněk po naředění.
Expand 1 Items
Glycin ≥99%
Supplier: Apollo Scientific
Component of tris-glycine-sds running buffers forpolyacrylamide gel electrophoresis.