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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® PerfectBlue Maxi ExW 'Revolution', Wide format gel system

VWR® PerfectBlue Maxi ExW 'Revolution', Wide format gel system

Supplier: VWR Collection

The Maxi ExW 'Revolution' offers built-in buffer recirculation - without the need for pumps or additional tubing. The Maxi ExW 'Revolution' offers the same high throughput, rapid separation as the Maxi ExW model but, with the position of the cathode and a rising tube, the system uses hydrogen bubbles to slowly mix the buffer. This system thereby avoids the build-up of pH or ionic gradients for electrophoresis of maximum quality and stability.

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VWR®, Accessories for PerfectBlue Vertical Gel Systems

VWR®, Accessories for PerfectBlue Vertical Gel Systems

Supplier: VWR Collection

Vertical gel electrophoresis system, accessory, Casting stand, Spare silicone rubber seals for upper buffer chamber/plate holders Cast-It M, Pro: PerfectBlue vertical gels

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VWR® Transfer Buffer Concentrate, CAPS 10X

VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR®, MOPS 10X buffer for biotechnology

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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VWR® A.C.E.™, Sequencing buffers

VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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Glycerin, anhydrous ≥99.5%, Ultrapure Bioreagent for biotechnology, for electrophoresis, J.T.Baker®

Glycerin, anhydrous ≥99.5%, Ultrapure Bioreagent for biotechnology, for electrophoresis, J.T.Baker®

Supplier: Avantor

Glycerol, anhydrous, Ultrapure Bioreagent, for enzyme stabilisation, freezing and electrophoresis buffers.

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VWR®, Tris-borate-EDTA (TBE) buffer

VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® PerfectBlue Mini L Revolution, Gel System

VWR® PerfectBlue Mini L Revolution, Gel System

Supplier: VWR Collection

The 'Revolution' series includes a buffer circulation system, powered by the electrodes themselves. Hydrogen bubbles produced at the cathode pass up a diagonally positioned pipe connected to the two sides of the tank producing a gentle circulation of the buffer. The result is no pH and ionic gradient – without need of mechanical pumps.

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VWR®, SSC buffer, 20X, ultra-pure grade

VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® Gentle ReView™, Stripping Buffer

VWR® Gentle ReView™, Stripping Buffer

Supplier: VWR Chemicals

Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.

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VWR® PerfectBlue Maxi M 'Revolution', Gel system

VWR® PerfectBlue Maxi M 'Revolution', Gel system

Supplier: VWR Collection

Based on the Maxi M model, the Maxi M 'Revolution' offers a built-in buffer circulation system, powered by the system’s electrodes. At the cathode, hydrogen bubbles are produced and pass up a diagonally positioned pipe connected to the two sides of the tank to produce a gentle and continual circulation of the buffer. The system nulls any pH and ionic gradient – without the fuss of mechanical pumps. The system offers 6 comb positions enabling the gel to be partitioned into 2, 3 or 4 areas. In addition, the system can accommodate any of the 23 standard and microtitre comb designs described for the Maxi M model.

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR®, Red Taq DNA polymerase master mix

VWR®, Red Taq DNA polymerase master mix

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate

VWR® Phosphate Buffered Saline (PBS), Low Phosphate

Supplier: VWR Chemicals

Irradiated solution for use in washing cells.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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2X SDS-PAGE sample buffer without DTT or b-ME

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

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VWR® Separating Gel Buffer, 4X

VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR® Phosphate Buffered Saline (PBS), High Phosphate

VWR® Phosphate Buffered Saline (PBS), High Phosphate

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

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Peqlab PerfectBlue Maxi M 'Revolution', Gel system

Peqlab PerfectBlue Maxi M 'Revolution', Gel system

Supplier: VWR Peqlab

Based on the Maxi M model, the Maxi M 'Revolution' offers an in-built buffer circulation system, powered by the system's electrodes. At the cathode, hydrogen bubbles are produced and pass up a diagonally positioned pipe connected to the two sides of the tank to produce a gentle and continual circulation of the buffer. The system nulls any pH and ionic gradient – without the fuss of mechanical pumps. The system offers 6 comb positions enabling the gel to be partitioned into 2, 3 or 4 areas. Additionally, the system can accommodate any of the 23 standard and microtitre comb designs described for the Maxi M model.

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VWR® UltraPure, PBS and TBS wash buffers

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR® EZ-Vision®, DNA Ladder, 100 bp

VWR® EZ-Vision®, DNA Ladder, 100 bp

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

    
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Electrophoresis running buffers

Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

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VWR®, Loading dye base

VWR®, Loading dye base

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

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VWR® Proteomics Grade, Tris-glycine buffer

VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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VWR®, fyziologický roztok pufrovaný fosfátem (PBS), nízký obsah fosfátů, 10X

VWR®, fyziologický roztok pufrovaný fosfátem (PBS), nízký obsah fosfátů, 10X

Supplier: VWR Chemicals

Ozářený koncentrát PBS, vhodný pro použití při promývání buněk po naředění.

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Glycin ≥99%

Supplier: Apollo Scientific

Component of tris-glycine-sds running buffers forpolyacrylamide gel electrophoresis.

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