Blue light transilluminator, TruBlu™
Supplier: EDVOTEK
The TruBlu™ blue light transilluminator is ideal for viewing DNA gels stained with SYBR® Safe, thus eliminating the need for UV light or ethidium bromide. It is optimised to fit Edvotek® gels as well as any other agarose gels.
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Experimentation kits, DNA fingerprinting - using PCR
Supplier: EDVOTEK
Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.
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White light box
Supplier: EDVOTEK
The Edvotek White Light Box features a spacious 25×25 cm viewing area illuminated by long life LEDs and is housed in a slim aluminium body. It is designed to safely enhance the visualisation of DNA stained with FlashBlue™, proteins stained with Coomassie Blue and autoradiograms.
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Floating racks
Supplier: EDVOTEK
Floating rack is ideal for floating tubes in water baths and tubs.
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SYBR® Safe Stain
Supplier: EDVOTEK
SYBR® Safe Stain is a DNA stain that fluoresces with a bright green colour when excited with UV light. Like ethidium bromide, SYBR® Safe Stain binds specifically to the DNA double helix.
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Urinalysis Using Simulated Urine
Supplier: EDVOTEK
Urinalysis is one of the oldest medical diagnostic tests, and is still one of the most commonly used tests to this day.
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Kits: restriction enzyme cleavage patterns of DNA, Ready-to-Load™
Supplier: EDVOTEK
Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.
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Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes
Supplier: EDVOTEK
The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
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Tris-Glycine buffer
Supplier: EDVOTEK
For vertical polyacrylamide gel electrophoresis.
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Experimentation kits, Mini-Prep isolation of plasmid DNA
Supplier: EDVOTEK
Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.
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Experimentation kits, principles of enzyme catalysis
Supplier: EDVOTEK
This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.
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Coloured beads
Supplier: EDVOTEK
A set of coloured beads that can be designated to represent the Watson-Crick DNA bases: A, T, G, C.
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Experimentation kits, antigen-antibody interaction: the ouchterlony procedure
Supplier: EDVOTEK
This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.
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Kits: in search of my father, Ready-to-Load™
Supplier: EDVOTEK
Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.
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Experimentation kits, simulation of HIV detection by ELISA
Supplier: EDVOTEK
An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.
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Classroom molecular biology toys and games
Supplier: EDVOTEK
Classroom molecular biology toys and games
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Single channel pipettes, fixed volume, MiniPipettes
Supplier: EDVOTEK
Micropipettes which use standard pipette tips. No need to calibrate and near impossible to measure the wrong volume.
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Experimentation kits, introduction to plant cell culture
Supplier: EDVOTEK
Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.
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Experimentation kits, PCR-based Alu-Human DNA typing
Supplier: EDVOTEK
Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.
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Experimentation kits, blood typing
Supplier: EDVOTEK
ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.
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Transformation with green fluorescent protein
Supplier: EDVOTEK
In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
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Experimentation kits, southern blot analysis
Supplier: EDVOTEK
This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
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Ready-to-Load™ Kits, PCR
Supplier: EDVOTEK
Ready-to-Load™ PCR kit introduces students to the principles and applications of the Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler.
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Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants
Supplier: EDVOTEK
Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
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Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™
Supplier: EDVOTEK
Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.