Hledali jste: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Dulbecco's phosphate-buffered saline (DPBS), Corning®
Supplier: Corning
Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7,0 to 7,6
Expand 10 Items
Phosphate-buffered saline (PBS), Corning®
Supplier: Corning
Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate
Expand 3 Items
Hank's balanced salt solutions (HBSS), Corning®
Supplier: Corning
Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.
Expand 10 Items
Phosphate-Buffered saline (PBS), 10X concentrate, pH 7,4, molecular biology grade, Corning®
Supplier: Corning
Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research
Expand 1 Items
MOPS buffer, powder, Corning®
Supplier: Corning
Running Buffer, MOPS buffer, powder, 100 g
Expand 1 Items
TE buffer, 1X, liquid, Corning®
Supplier: Corning
Tris-EDTA (TE) buffer is commonly used in work with nucleic acids.
Expand 1 Items
Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
Expand 1 Items
10X TBE buffer, liquid, Corning®
Supplier: Corning
TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis.
Expand 1 Items
Tris base buffer, powder, Corning®
Supplier: Corning
Tris is commonly used in buffer solutions in biochemical and molecular biological research.
Expand 3 Items
Buffer, HEPES solution (1 mol/L), Corning®
Supplier: Corning
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2
Expand 1 Items
Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
Expand 3 Items
10X TAE buffer, liquid, Corning®
Supplier: Corning
Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer
Expand 1 Items
20X SSPE, liquid, Corning®
Supplier: Corning
20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.
Expand 1 Items
10X Tris buffered saline, liquid, Corning®
Supplier: Corning
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.
Expand 1 Items
1M tris-hydrochloride buffers, Corning®
Supplier: Corning
Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.
Expand 2 Items
Sodium acetate, Corning® 3M™
Supplier: Corning
Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.
Expand 1 Items
10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
Expand 1 Items
EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.