171728 Results for: "Blotting"

Supplier: Thermo Fisher Scientific

Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.

Supplier: Thermo Fisher Scientific

SuperSignal® ELISA Femto maximum sensitivity substrate utilises the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications. Ideal for automated high-throughput screening applications.

Supplier: Thermo Fisher Scientific

The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.

Supplier: Thermo Fisher Scientific

QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Thermo Fisher Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

Supplier: EDVOTEK

The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.

Supplier: Whatman products (Cytiva)

Membrány Protran® jsou vyrobeny ze 100% čisté nitrocelulózy, aby byla zajištěna nejvyšší možná vazebná kapacita. Jsou kompatibilní s řadou detekčních metod, včetně izotopových, chemiluminiscenčních, kolorimetrických a fluorescenčních. Není vyžadován žádný krok předmáčení methanolem, což z této membrány dělá volbu pro proteiny, které preferují vodné prostředí. Před přenosem je membrána jednoduše navlhčena vodou a poté umístěna do přenosového pufru. Žádné další kroky předběžného ošetření nejsou nutné.

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

Supplier: G-Biosciences

A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.

Supplier: Thermo Fisher Scientific

SuperBlock Blocking buffer is an optimised solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.

Supplier: BOEKEL SCIENTIFIC

Rocker platform for incubation of large blots in flat-bottomed containers

Supplier: G-Biosciences

A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

Supplier: Rockland Immunochemicals

IP Western blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing interference.

Supplier: MILLIPORE

Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.

Supplier: Whatman products (Cytiva)

Cellulose Chromatography and Blotting Paper Roll contains cotton linter filter sheets for electrophoresis and amino acid chromatography.

Supplier: Whatman products (Cytiva)

Membrána Nytran® Nylon (Nytran® N) je ideální pro aplikace vyžadující nižší náboj. Je navržena pro Southernův a Northernův blotting, stejně jako přenos kolonií a plaku a "Dot Blots" nebo "Slot Blots" techniky. Nytran® N je kompatibilní s izotopními detekčními metodami.

Supplier: MILLIPORE

These Immobilon® PVDF membranes, each optimised for different protein blotting applications.

Supplier: VWR Collection

Minitřepačka v sobě kombinuje vlastnosti orbitální a výkyvné třepačky tak, že vytváří 3D pohyb, který je dostatečně šetrný pro křehké gely a blotovací destičky, avšak zajišťuje důkladné promíchávání.

Supplier: Cytiva

The simple, modular design of miniVE vertical electrophoresis system consolidates all the small parts for electrophoresis and blotting into one convenient unit. Uniform gel temperature is maintained with the tank-style lower buffer chamber. A full tank stabilises the temperature so that lanes are straight and bands are flat. The blot module will accept two gel/membrane stacks simultaneously. The lower buffer chamber is designed to hold two blot modules, allowing four gels to be blotted at once.

Supplier: Abcam

Anti-SDHA+COX1+Beta-Actin Antibody

Supplier: LONZA

Precast gel, Precast agarose IEF plate accessory bulk pack, contains 125 mm wicks and blotting paper (100 of each)

Supplier: Abcam

Anti-ATP5A+GAPDH+Histone H3 Antibody

Supplier: Abcam

Anti-Sodium Potassium ATPase+GAPDH+Histone H3 Antibody

Supplier: Abcam

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Supplier: Merck

Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Supplier: Whatman products (Cytiva)

Nylonové membrány Nytran® SuPerCharge (SPC) mají velice vysoký kladný náboj a vyšší hustotu nylonu na jednotku plochy. Dostupné jako kruhové výseče, archy nebo role pro mikrodestičky.

Supplier: Abcam

Anti-Caspase-3 + Cleaved PARP1 + Muscle Actin Antibody

Supplier: MILLIPORE

Accessories for Protein Detection System

Supplier: Thermo Fisher Scientific

Pufr pro elektroforézu, Transfer buffer, Transfer Electroblot Buffer 10X