171728 Results for: "Blotting"
Cell extract HL60 (non-induced)
Supplier: ENZO LIFE SCIENCES
Whole-cell extract of human HL-60 leukemia cells.
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picoLUCENT™ PLUS-AP kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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Immobilon®-P Blotting Sandwich Transfer Membranes
Supplier: MILLIPORE
Immobilon®-P Blotting Sandwich Transfer Membranes
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Blotting membranes, Amersham™ Hybond™-N+
Supplier: Cytiva
Amersham™ Hybond™-N+ is a positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or non radioactive chemiluminescence and chemifluorescence detection systems.
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Blotting membranes, Amersham™ Hybond™ 0.2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ 0.2 PVDF is a 0,2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimises 'blow-through' and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of
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IHC chromogen substrate (AEC, stable), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.
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CYTAG™ CGH labelling kits
Supplier: ENZO LIFE SCIENCES
The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.
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SSC (20X), RNAse free
Supplier: Thermo Fisher Scientific
SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.
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Pierce™, Biodyne™ A Blotting Membranes, Nylon
Supplier: Thermo Fisher Scientific
Biodyne™ A nylon membranes are sheets of unmodified nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ A nylon membrane is manufactured with a matrix pore size of 0,45 micron. It contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules.
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Luminata™ Chemiluminescent ELISA HRP Substrates
Supplier: MILLIPORE
Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.
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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
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Blotting membranes, Amersham™ Hybond™ SEQ 0,2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ SEQ 0.2 PVDF is a 0,2 µm pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane minimises ‘blow-through’ and increases protein binding over a wide range of molecular weights.
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Semi Dry Blot Transfer buffer (10X)
Supplier: Thermo Fisher Scientific
Pufr pro elektroforézu, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X
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High molecular weight transfer buffer (5X)
Supplier: G-Biosciences
High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
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Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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Horseradish peroxidase, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
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Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
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Experimentation kits, southern blot analysis
Supplier: EDVOTEK
This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
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NPP ELISA alkaline phosphatase substrate
Supplier: Rockland Immunochemicals
NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.
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Caspase-3 substrates, NucView®
Supplier: Biotium
NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.
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HRP conjugates, HisProbe™
Supplier: Thermo Fisher Scientific
HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
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Immobilon™ Block Noise Canceling Reagents
Supplier: MILLIPORE
Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.
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Luminol and oxidising solutions
Supplier: Thermo Fisher Scientific
For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.
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Western Blot Transfer buffer, 10X, methanol-free, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.
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Amersham™ ECL Direct™ detekční činidla, Cytiva
Supplier: Cytiva
Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.
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Saponin permeating solution, 0,5% w/v solution in PBS (10X)
Supplier: Thermo Fisher Scientific
Saponin permeating solution, is used as a cell permeabilising agent.
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Pharmalytes® IEF carrier ampholytes
Supplier: Cytiva
Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
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Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X
Supplier: MILLIPORE
Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.
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Sample clean up, Perfect-FOCUS™
Supplier: G-Biosciences
Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.
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CheLuminate-HRP PicoDetect Chemiluminescent Detection Kit
Supplier: PanReac AppliChem
The CheLuminate-HRP PicoDetect is a complete kit with ready to use reagents for chemiluminescent detection of immobilised proteins (Western blot) or immobilised nucleic acids (Southern or Northern blot), conjugated with horseradish peroxidase (HRP) directly or indirectly.