171728 Results for: "Blotting"
picoLUCENT™ PLUS-AP kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
Expand 2 Items
Western blotting markers, Amersham™ ECL™ DualVue™
Supplier: Cytiva
Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.
Expand 1 Items
Immobilon®-P Blotting Sandwich Transfer Membranes
Supplier: MILLIPORE
Immobilon®-P Blotting Sandwich Transfer Membranes
Expand 2 Items
Blotting membranes, Amersham™ Hybond™-N+
Supplier: Cytiva
Amersham™ Hybond™-N+ is a positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or non radioactive chemiluminescence and chemifluorescence detection systems.
Expand 8 Items
IHC chromogen substrate (AEC, stable), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.
Expand 1 Items
Blotting membranes, Amersham™ Hybond™ 0.2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ 0.2 PVDF is a 0,2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimises 'blow-through' and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of
Expand 5 Items
CYTAG™ CGH labelling kits
Supplier: ENZO LIFE SCIENCES
The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.
Expand 2 Items
SSC (20X), RNAse free
Supplier: Thermo Fisher Scientific
SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.
Expand 2 Items
Pierce™, Biodyne™ A Blotting Membranes, Nylon
Supplier: Thermo Fisher Scientific
Biodyne™ A nylon membranes are sheets of unmodified nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. Biodyne™ A nylon membrane is manufactured with a matrix pore size of 0,45 micron. It contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules.
Expand 1 Items
Luminata™ Chemiluminescent ELISA HRP Substrates
Supplier: MILLIPORE
Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.
Expand 3 Items
Blotting membranes, Amersham™ Hybond™ SEQ 0,2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ SEQ 0.2 PVDF is a 0,2 µm pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane minimises ‘blow-through’ and increases protein binding over a wide range of molecular weights.
Expand 1 Items
Semi Dry Blot Transfer buffer (10X)
Supplier: Thermo Fisher Scientific
Pufr pro elektroforézu, Transfer buffer, Semi-dry Blot Transfer Buffer, 10X
Expand 2 Items
Horseradish peroxidase, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
Expand 1 Items
High molecular weight transfer buffer (5X)
Supplier: G-Biosciences
High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
Expand 1 Items
Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
Expand 8 Items
Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
Expand 3 Items
VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
Expand 1 Items
Experimentation kits, southern blot analysis
Supplier: EDVOTEK
This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
Expand 1 Items
HRP conjugates, HisProbe™
Supplier: Thermo Fisher Scientific
HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
Expand 1 Items
Immobilon™ Block Noise Canceling Reagents
Supplier: MILLIPORE
Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.
Expand 3 Items
NPP ELISA alkaline phosphatase substrate
Supplier: Rockland Immunochemicals
NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.
Expand 3 Items
Caspase-3 substrates, NucView®
Supplier: Biotium
NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.
Expand 12 Items
Luminol and oxidising solutions
Supplier: Thermo Fisher Scientific
For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.
Expand 2 Items
Western Blot Transfer buffer, 10X, methanol-free, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.
Expand 1 Items
BCIP®/NBT alkaline phosphatase substrate, SIGMAFAST™
Supplier: Merck
SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.
Expand 2 Items
Peroxidase conjugate stabiliser/diluent, Guardian™
Supplier: Thermo Fisher Scientific
Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.
Expand 2 Items
Amersham™ ECL Gold Hybridization Buffer, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Expand 1 Items
Nucleic acid labelling and detection system, AlkPhos Direct™
Supplier: Cytiva
AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
Expand 3 Items
Sample clean up, PAGE-Perfect™
Supplier: G-Biosciences
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.
Expand 2 Items
Rehydration reagent, DeStreak
Supplier: Cytiva
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.