"Biowest"

Supplier: Biowest

Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Supplier: Biowest

Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

Supplier: Biowest

Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: Biowest

Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

Supplier: Biowest

Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Supplier: Biowest

Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

Supplier: Biowest

Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.

Supplier: Biowest

Gentamycin sulphate acts against Gram-negative, Gram-positive bacteria and mycoplasma.

Supplier: Biowest

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: Biowest

Bovine serum is a pooled serum sample obtained from a normal donor herd. It is specific for anti-bovine serum and yields multiple arcs of precipitation during immunoelectrophoresis versus anti-bovine serum.

Supplier: Biowest

Sterile filtered foetal bovine serum (FBS) derived from clotted whole blood aseptically collected from foetus via cardiac puncture. Each manufactured batch is rigorously controlled, from the collection of serum through to final packaging.

Supplier: Biowest

Bovine serum albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other components of serum are unwanted. In cell culture, its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger to remove toxic substances, including pyrogens, from the medium.