Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulphate. It has also been used as a Azocasein substrate for the determination of proteolytic activity.

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen.