Ascentis®Phenyl HPLC columns are optimised to the three terms of the resolution equation: efficiency, retention, and selectivity.

• High purity, type B silica for inertness, reproducibility, and stability
• Modern bonding process optimises bonded phase coverage and maximises stability, while minimising bleed and unwanted secondary interactions
• Compatible with LC-MS and all of today's sensitive instruments and methods
• Scalable sensitivity from analytical to preparative
• High surface area silica for high preparative loading capacity
• Impurity: <5 ppm metals
• Surface coverage: 5,2 µmol/m²

Ascentis® Phenyl phases are n-basic (electron donating) and are similar in overall retention to alkyl and EPG phases for easy column screening. The alternative selectivity of phenyl phases is often explained by the n-n interactions available through the phenyl ring. They provide low-bleed for MS or UV gradient applications due to the use of trifunctional bonding reagent, outstanding phenyl selectivity due to high phase loading and short butyl spacer and 100 % aqueous-compatible for highly-polar compounds.

Ascentis® bonded phases have a wide range of selectivities. It is likely that one or more Ascentis® phase will accomplish any small molecule HPLC separation. Ascentis® products cover all HPLC application areas, including the most sensitive trace-level analyses.