This mAb recognises a protein of ~50 kDa identified as SOX10. This mAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Western blot: 0,5 - 1 µg/ml Immunohistochemistry (FFPE): 0,5 - 1 µg/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Optimal dilution of the SOX10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: SOX10
Clonality: Monoclonal
Clone: SOX10/991
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human, Mouse