T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.

• Product source: E. coli cells expressing T4 gp32 gene
• Storage temperature: −20 °C
• Heat inactivation: 65 °C for 20 min
• Quality control assays: T4 GP32 protein is free from detectable nuclease contamination

Product includes: T4 GP32 protein and 10X GP32 reaction buffer

10X GP 32 reaction buffer composition:
200 mM Tris-acetate
1M Potassium acetate
100 mM Magnesium acetate
10 mM DTT
pH 7,8 at 25 °C