About this item
Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degredation of the full length FGF-basic N-terminus results in a truncated FGF-basic 147aa protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-basic within the cell, but do not affect the biological activity of FGF-basic. Therefore, there are no detectable differences in biological activity between the full length FGF-basic 154aa and the truncated FGF-basic 147aa recombinant proteins.
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- Low Endotoxin
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A COA is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions. This product is for research use only and is not for use in humans.
Specifications
- Spojení:Unconjugated
- Proteinový podtyp:Cytokine
- Typ proteinu/peptidu:Recombinant
- Zdroj:E. coli
- Druhy:Human
- Podmínky uskladnění:–20 °C
- Obsah endotoxinů:Endotoxin LAL (EU/µg) ≤0,1
- Biologická aktivita:Bioactivity assay: NR6R-3T3 cell proliferation, ED50 ≤5 ng/ml, Bioactivity ≥2,0×10⁵ units/mg
- Identifikační číslo genu:P09038
- Pokyny pro rekonstituce:Sterile water at 0.1 mg/ml
- Bez obsahu endotoxinů:N
- Bez nosiče:Y
- Bez obsahu proteázy:N
- Bez obsahu živočichů:Y
- Proteinová synonyma:Prostatropin|HBGF-2|FGF2
- Název proteinu/peptidu:FGF-basic 147
- Čistota:≥95%
- Molekulární hmotnost:16,5 kDa
- Sekvence:MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
- Úroveň endotoxinů:Low
- Formulace:10 mM sodium phosphate, 75 mM sodium chloride, pH 7,5
- Bez nukleáz:N
- Třída:RUO
- Transportní teplota:RT
- Testované aplikace:Bioassay