Aflatoxins are mycotoxins that are generated as a result of secondary metabolism of various fungal species such as Aspergillus, Penicillium and Fusarium.

Aflatoxin B₂ can be used as a reference standard for the determination of aflatoxin B₂ in animal feed by high performance liquid chromatography with fluorescence detection (HPLC-FLD), as well as quick, easy, cheap, effective, rugged and safe (QuEchERS) extraction method combined with ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS), in eggs by QuEchERS extraction method followed by analysis using UHPLC with tandem mass spectrometry (MS/MS) operating on electrospray-ionization interface (ESI) mode, in Brazil nuts by immunoaffinity column (IAC) and clean-up HPLC-FLD and in plant-based milk and dairy products by dispersive liquid-liquid microextraction (DLLME), QuEchERS extraction method and HPLC-FLD.