3-Acetyldeoxynivalenol belongs to the class of trichothecenes, which are secondary metabolites generated by various fungi, particularly by Fusarium species.

3-Acetyldeoxynivalenol may be used as an analytical reference standard for the determination of the analyte in: Cereals by gas chromatography-mass spectrometry (GC-MS), milk samples by liquid chromatography combined with tandem mass spectrometry (LC-MS/MS).