Highly active

Produced in insect cells.

Approximately 1:1 stoichiometric complex of recombinant human HDAC3 (histone deacetylase 3) with a GST fusion protein comprising the Deacetylase Activation Domain (DAD) of human NCOR1 (amino acids 397-503; SANT1 domain plus amino flanking residues). Produced by coexpression of HDAC3 with a GST-NCOR1(DAD) construct in an insect cell system, the complex displays ~100-fold higher deacetylase activity than isolated HDAC3 (based on equal weights HDAC3 assayed at a saturating concentration (100µM) of a fluorogenic peptide substrate p53 379-382, K(Ac)382; Prod. No. BML-KI177).This HDAC3 preparation is active with various fluorogenic peptide substrates, including Fluor de Lys™ Substrate (Prod. No. BML-KI104) and Fluor de Lys™-SIRT1 (p53 379-382, K(Ac)382; Prod. No. BML-KI177).