K. pneumoniae Recombinant Kanamycin kinase type II (from E. coli)
PRSI92-658
:
- Bal.:50 µG
- Typ enzymu:Recombinant
- Zdroj:E. coli
- Druhy:K. pneumoniae
- Podmínky uskladnění:Lyophilized protein should be stored at –20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4...7 °C for 2 - 7 days. Aliquots of reconstituted samples are stable at –20 °C for 3 months.
- Název enzymu:Kanamycin kinase type II
- Čistota:>95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:29 kDa
- Sekvence:Met1-Phe264
- Formulace:Lyophilized from a 0,2 µm filtered solution of PBS, pH 7,4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.
This recombinant protein can be used for biological assays. For research use only.