S. hygroscopicus recombinant PPT N-acetyltransferase (from E. coli)
PRSI92-657
:
- Bal.:50 µG
- Typ enzymu:Recombinant
- Zdroj:E. coli
- Druhy:S. hygroscopicus
- Podmínky uskladnění:Lyophilized protein should be stored at –20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4...7 °C for 2 - 7 days. Aliquots of reconstituted samples are stable at –20 °C for 3 months.
- Název enzymu:PPT N-acetyltransferase
- Čistota:>95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:20,6 kDa
- Sekvence:Met1-Ile183
- Formulace:Lyophilized from a 0,2 µm filtered solution of PBS, pH 7,4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
This recombinant protein can be used for biological assays. For research use only.