Human recombinant Isocitrate Dehydrogenase (from E. coli)
PRSI92-613
:
- Bal.:50 µG
- Typ enzymu:Recombinant
- Zdroj:E. coli
- Druhy:Human
- Sekvence značek:C-8 His tag
- Podmínky uskladnění:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Název enzymu:Isocitrate Dehydrogenase
- Čistota:>95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:48.1 kDa
- Sekvence:Met1-Leu414
- Formulace:Supplied as a 0,2 µm filtered solution of 50 mM Tris, 200 mM NaCl, 10% glycerol, pH 8,0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer, binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.
Fusion-Tag: C-8 His tag
This recombinant protein can be used for biological assays. For research use only.