- Bal.:50 µG
- Typ enzymu:Recombinant
- Druhy:Mouse
- Sekvence značek:C-6 His tag
- Podmínky uskladnění:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Název enzymu:Carbonic anhydrase 14
- Čistota:>95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:31.8 kD
- Sekvence:Ala16-Met290
- Formulace:Supplied as a 0.2 µm filtered solution of 20 mM Tris, 150 mM NaCl,pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Mouse Ca14,also known as Carbonic anhydrase 14,is a member of large family of zinc metalloenzymes. It could catalyse reversible hydration of carbon dioxide. The reaction is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Fifteen CA isoforms have been reported so far. They have different patterns of tissue-specific expression and physiologic roles. Some CAs may serve as markers for tumours and hypoxia. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C- terminal segment with several potential phosphorylation sites. A subset of CAs lack CA activity due to point mutations but retain esterase function. CA14 is widely expressed in the central nervous system.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.