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Human recombinant lysine--tRNA ligase (from cells)
  PRSI91-664
 :  
Human recombinant lysine--tRNA ligase (from cells)
  PRSI91-664
 :  

 

  • Bal.:
    50 µG
  • Typ enzymu:
    Recombinant
  • Zdroj:
    Cells
  • Druhy:
    Human
  • Sekvence značek:
    C-6 His tag
  • Podmínky uskladnění:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Název enzymu:
    Lysine--tRNA Ligase
  • Čistota:
    >95% as determined by reducing SDS-PAGE
  • Molekulární hmotnost:
    69.1 kD
  • Sekvence:
    Ala2-Val597
  • Formulace:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl,1 mM DTT, 20% glycerol,pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Testované aplikace:
    Bioassay

 

 

Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localisation, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerisation and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyses the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.