- Bal.:50 µG
- Typ enzymu:Recombinant
- Zdroj:Cells
- Druhy:Human
- Sekvence značek:C-6 His tag
- Podmínky uskladnění:Lyophilized protein should be stored at –20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4...7 °C for 2 - 7 days. Aliquots of reconstituted samples are stable at –20 °C for 3 months.
- Název enzymu:Carboxypeptidase A2
- Čistota:>95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:45.91 kD
- Sekvence:Leu17-Tyr417
- Formulace:Lyophilized from a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Testované aplikace:Bioassay
Carboxypeptidase A2 (CPA) is a secreted pancreatic procarboxy-peptidase that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.