Human recombinant Flap Endonuclease 1 (from E. coli)
Catalog # PRSI91-123
Supplier: ProSci Inc.
CAS Number:
Specifications
- Bal.:50 µG
- Typ enzymu:Recombinant
- Zdroj:E. coli
- Druhy:Human
- Podmínky uskladnění:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Název enzymu:Flap Endonuclease 1
- Čistota:>95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:42.59 kD
- Sekvence:Met1-Lys380
- Formulace:Supplied as a 0.2 µm filtered solution of 50 mM Tris, 50 mM NaCl, 1 mM DDT, 10% Glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Testované aplikace:Bioassay
Specifications
About this item
Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
This recombinant protein can be used for biological assays. For research use only.