Specifications
- Bal.:50 µG
- Typ proteinu/peptidu:Recombinant
- Zdroj:E. coli
- Druhy:Human
- Sekvence značek:C-6 His tag
- Podmínky uskladnění:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
- Proteinová synonyma:IP17|Ubiquitin-like Protein ISG15|Interferon-Induced 15 kDa Protein|hUCRP|ISG15|UCRP|Interferon-Induced 17 kDa Protein|G1P2|Ubiquitin Cross-Reactive Protein
- Název proteinu/peptidu:Interferon-stimulated Gene 15
- Čistota:> 95% as determined by reducing SDS-PAGE
- Molekulární hmotnost:18.2 kD
- Sekvence:Gly2-Gly157
- Formulace:Supplied as a 0.2 um filtered solution of 50mM HEPES, 100mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Testované aplikace:Bioassay
Specifications
About this item
Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signalling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localises with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.
- This recombinant protein can be used for biological assays. For research use only.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.