Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.

• Used for DNA labeling
• Not for 5´→3´ or 3´→5´exonuclease activity
• N-terminal truncated DNA Polymerase I which retains polymerase activity

It is formulated of liquid in 20 mM TRIS-HCl, pH 7,5, containing 50% glycerol, 0,1 mM EDTA and 1 mM DTT.