E. coli

Aliquot and store at −20 °C or −80 °C. Avoid repeated freeze/thaw cycles.

C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.

Activator

c-Jun

Greater than 95% as determined by SDS-PAGE.

It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to concentration of 0.2-1.0 mg/ml.

C-JUN is supplied as lyophilized powder containing no additives.

SDS-PAGE , HPLC , FuncS