100 µG

Store at−20 °C.

This Leptin triple mutant is capable of inhibiting leptininduced proliferation of BAF/3 cells stably transfectedwith the long form of human leptin receptor. It also inhibitsvarious leptin effects in several in vitro bioassays.

LEP

Leptin

95% pure by SDS-PAGE

It is recommended to reconstitute the lyophilized Leptinmutant in sterile 0.4% NaHCO3 adjusted to pH 8-9, notless than 100 g/ml, which can then be further diluted toother aqueous solutions. At low concentrations additionof a carrier protein (0.1% HSA or B

16 kDa

Lyophilized. Leptin is lyophilized from a solution containing 0.003 mM NaHCO3.