The Pierce mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimise and confirm correct operation of their equipment.

• Assess chromatography and MS instrument performance
• Predict peptide retention across multiple instrument platforms
• Predict peptide retention time from sequence using calculated hydrophobicity factor
• Optimise scheduled MS acquisition windows for improved quantification and increased multiplexing
• Normalise collision energies between instruments and platforms
• Normalise results for variation in retention times and peak intensities between runs

Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalise LC-MS performance between samples and over time.