The Pierce™ Human Rhinovirus (HRV) 3C Protease is a recombinant cysteine protease used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

• Highly specific with no off-target cleavage even at high enzyme/substrate concentration
• Allows cleavage reaction to take place at 4°C without affecting the functionality of purified protein
• Can be used either following affinity purification or while fusion proteins are bound to glutathione or a tag-specific resin
• Dual-tagged enzyme is compatible with various columns and buffers

The HRV 3C protease enzyme (47,8 kDa) is genetically engineered and contains both GST and 6×His tags so that it can be removed by Ni, cobalt or glutathione (GSH) resin. It is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue. This is a restriction grade protease that has robust activity at 4 °C.