The DyLight™ Antibody Labeling Kits were specifically developed for fast, efficient labeling of antibodies. Two convenient kit formats are available to accommodate varied labeling requirements. The DyLight™ Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.

• Fast – fluorescently label and purify protein in approximately one hour
• Amine-reactive dyes – label virtually any protein
• Pre-measured fluorescent dye – eliminate the time, waste and hassle associated with weighing dye
• Efficient non-reacted dye removal
• Minimal sample dilution
• Spin column format eliminates the need for column preparation, fraction screening and waiting for protein to emerge from column
• Easy protocol

The DyLight™ Microscale Antibody Labeling Kits contain all the necessary components to perform five separate labeling reactions using 100 μg of IgG. The labeling kits use high-performance spin desalting columns to provide exceptional dye removal and antibody recovery.

Invitrogen DyLight™ Antibody Labeling kits enable the creation of fluorescent antibodies that are covalently linked to different DyLight™ fluorophores. The fluorescently labeled antibodies can be used in a wide variety of laboratory applications, including immunofluorescence microscopy, IHC, western blotting, ELISA, flow cytometry, or in vitro and in vivo fluorescent detection strategies.

DyLight™ dyes exhibit higher fluorescence intensity and photostability than CyDye and LI-COR™ dyes in many applications and remain highly fluorescent over a broad pH range (pH 4–9).

Chemical Reactivity: Amine
Labeling Target: Antibodies (General), Proteins (General)