Affinity chromatography columns, HiScreen™ Ni FF

HiScreen columns prepacked with Ni Sepharose 6 Fast Flow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

• High binding capacity, approx. 40 mg/ml medium and negligible leakage of Ni²⁺
• Prepacked, ready-to-use columns for convenient process development
• Excellent choice for method optimization and parameter screening due to the 10 cm bed height
• Easy connection in series to achieve 20 cm bed height
• Small bed volume gives fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening.

HiScreen columns have small bed volumes (4,7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose 6 Fast Flow which has high binding capacities for histidine-tagged proteins and minimal Ni²⁺ leakage.