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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Affinity coupling media, CNBr-Activated Sepharose™ 4B
  17-0430-01
 :  Cytiva
 :  
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
  17-0430-01
 :  Cytiva
 :  

 

  • Velikost částic:
    90 µm
  • Bal.:
    15 g
  • Popis:
    CNBr-activated Sepharose™ 4B
  • Sekvence:
    01
  • Rozsah pH:
    2 - 11
  • Matice:
    Agarose, 4%
  • Skladovací teplota:
    2 - 8 °C
  • Kapacita vázání:
    25 mg α-chymotrypsinogen/ml medium-60 mg α-chymotrypsinogen/ml
  • Typ:
    Solid

 

 

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

  • Multipoint attachment resulting in a chemically stable product
  • Over 25 years proven track record for coupling ligands containing primary amino groups
  • The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out
  • Stable to all commonly used aqueous solutions
  • Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride
  • Stable in organic solvents, such as 50% dimethylformamide and 50% dioxane

It provides a very convenient way to immobilise ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilisation of proteins, peptides and nucleic acids. For immobilising antibodies or other large proteins containing -NH₂ groups by coupling them to the matrix.