Supplier: Novus Biologicals

BACH2 Overexpression Lysate (Adult Normal)

Supplier: ProSci Inc.

Thymus tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Kidney tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Brain tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Eye tissue lysate (0 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells.

Supplier: PROVITRO

HKER-d - Human keratinocytes from dermis - are obtained from human tissue, in particularly from the Dermis, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HKER cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: PROVITRO

Our ImmunoSafe-HLA I and ImmunoSafe-HLA II panels are meticulously designed to enhance the predictivity of your preclinical assays. With 10 HLA Class I- or HLA Class II-matched donors, these panels can be tailored to suit your specific assay requirements. By providing a comprehensive resource to mimic diverse immunological responses, they empower researchers to achieve more accurate and relevant experimental outcomes.

Supplier: PROVITRO

Human peripheral blood mononuclear cells (PBMCs) are utilised as source material for isolating lymphocytes (T cells, B cells and NK cells) and monocytes in research and across fields such as immunology, immuno-oncology, and cell and gene therapies.
All PBMCs are isolated through Ficoll density gradient separation from freshly obtained leukapheresis ACD-A packs and directly cryopreserved.

Supplier: Charles River Laboratories Cell Solutions

CD34⁺ cells are isolated using positive immunomagnetic cell separation procedures from umbilical cord blood. Human cord blood is collected aseptically at the time of delivery from healthy pregnant women under institutional review board (IRB) approved procedures.

Supplier: Charles River Laboratories Cell Solutions

CD34 depleted mononuclear cells from mobilised peripheral blood are isolated by depleting the CD34 cells using immunomagnetic anti-CD34 microbeads.

Supplier: Charles River Laboratories Cell Solutions

Bone marrow is a major source of stem cells, which are being used in cancer research, cell studies, transplantation studies, and other research.

Supplier: Charles River Laboratories Cell Solutions

Human peripheral blood M2 macrophages are derived from purified monocytes. CD14⁺ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of M-CSF for 10 days. The M2 phenotyping was confirmed by morphology and the expression of specific biomarkers which monitored by flow cytometry. The cells were harvested after 10 days of in vitro culture.

Supplier: Enzo Life Sciences

The PC-12 cell line was derived from a transplantable rat adrenal pheochromocytoma. These cells were maintained in Ham's F12K medium with L-glutamine (2 mM) adjusted to contain 1,5 g/L sodium bicarbonate (82,5%), horse serum (15%) and faetal bovine serum (2,5%).

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: Novus Biologicals

iNOS Overexpression Lysate (Adult Normal)

Supplier: Enzo Life Sciences

The HeLa cell line derived from a human donor's epithelioid carcinoma tissue (cervix).

Supplier: PROVITRO

Dental Pulp Stem Cells (DPSCs) are primarily sourced from the pulp tissues of deciduous teeth, primary incisors, and permanent third molars. The dental pulp, especially from third molars, is noted for its rich reservoir of stem cells. DPSCs derived from third molars express key pluripotency-associated transcription factors including oct4, sox2, klf4, nanog, and c-myc.

Supplier: PROVITRO

Bone Marrow CD34⁺ cells, enriched with CD34⁺ markers, are pivotal for haematopoiesis and immune modulation. These unique cells play a crucial role in hematopoietic stem cell transplantation and immune reconstitution. They contribute to the formation of various blood cell lineages, including immune effectors such as natural killer cells and cytotoxic T-cells, shaping both the adaptive and functional aspects of the immune system.

Supplier: PROVITRO

HUVEC-p - Human Umbilical Vein Endothelial Cells, pooled - are obtained from human tissue, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation. It's crucial to note that HUVEC-p cultures undergo careful methods during isolation, ensuring that they remain untransformed and unmutated, maintaining characteristics reflective of their in vivo state. These primary cultures have a limited in-vitro lifespan.

Supplier: ProSci Inc.

Eye tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Stomach tissue lysate (0 Day Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Stomach tissue lysate (14 Day Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Lung tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Heart tissue lysate (0 day old mouse) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: ProSci Inc.

Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin

Supplier: ProSci Inc.

Spleen tissue lysate (14 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

Supplier: Novus Biologicals

Flt3/CD135 Lysate (Adult Normal)

Supplier: PROVITRO

HCASMC - Human coronary artery smooth muscle cells - are obtained from human tissue, in particularly from the coronary artery smooth muscle cells, initially isolated in their in vivo state. The cells are then transitioned into a primary cell culture and subsequently aliquoted and frozen for preservation.