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Hledali jste: HPLC sorbenty

HPLC sorbenty

By pumping pressurized liquid solvent with the sample mixture through a HPLC sorbent-filled column, researchers are able to identify each mixture component using retention time measurements. These sorbents are capable of working with small particle sizes requiring high operational pressure, a common high-performance liquid chromatography operation characteristic. Able to separate and quantify, the HPLC sorbents have a hydrophobic or polar nature. The versatile materials function with digital microprocessing instruments for rapid testing.

Ion exchange resins, Diaion® HP20

Supplier: Thermo Fisher Scientific

Diaion® HP20 is used as a filler in chromatography columns due to its extremely large pores. It is considered as an effective adsorbent to eliminate toxic effects in fermentation and separation of traces heavy metal ions. It is also useful for the purification of peptides, proteins, fermentation products, polyphenols and cephalosporin C. It helps to remove bitter tasting substances from fruit juices.

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Ion exchange resins, Diaion® PA312 (Cl)

Supplier: Thermo Fisher Scientific

These resins are ideal for use in decolourisation in sugar solutions, metal recovery, pharmaceuticals and purification of amino acids.

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Anion exchange resins, Toyopearl® and TSK-GEL®

Anion exchange resins, Toyopearl® and TSK-GEL®

Supplier: TOSOH Bioscience

Anion exchange resins are polymer-based media developed for the separation of biomolecules including monoclonal antibodies and oligonucleotides. Several of these resins are available in multiple particle sizes allowing for their use in capture, intermediate purification or polishing process steps.

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Cation exchange resins, Toyopearl® and TSK-GEL®

Cation exchange resins, Toyopearl® and TSK-GEL®

Supplier: TOSOH Bioscience

Cation exchange resins are polymer based media developed for the separation of biomolecules including monoclonal antibodies and peptides.

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Affinity chromatography resins, Toyopearl®, TSK-GEL®

Affinity chromatography resins, Toyopearl®, TSK-GEL®

Supplier: TOSOH Bioscience

Affinity chromatography resins are functionalised with chemically active groups or group-specific ligands.

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Cation exchange bulk packing material

Cation exchange bulk packing material

Supplier: HAMILTON BONADUZ

Polymer bulk packing material for cation exchange applications.

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Ion exclusion bulk packing material

Ion exclusion bulk packing material

Supplier: HAMILTON BONADUZ

Polymer bulk packing material for ion exclusion applications.

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Hydrophobic interaction chromatography resins, Toyopearl®, TSK-GEL®

Hydrophobic interaction chromatography resins, Toyopearl®, TSK-GEL®

Supplier: TOSOH Bioscience

These are bulk media products which contain three or four different ligand types offered for a chromatography mode.

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Hydrophobic interaction chromatography resins, Toyopearl®, TSK-GEL®

Hydrophobic interaction chromatography resins, Toyopearl®, TSK-GEL®

Supplier: TOSOH Bioscience

Hydrophobic interaction chromatography (HIC) is a powerful tool for the process purification of biomolecules. Five HIC ligands featuring different degrees of hydrophobicity and selectivity. The hydrophobicity of HIC resins increases through the ligand series.

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Derivatisation reagent, Fluoraldehyde™ o-phthaldialdehyde, Pierce™

Derivatisation reagent, Fluoraldehyde™ o-phthaldialdehyde, Pierce™

Supplier: Thermo Fisher Scientific

Fluoraldehyde crystals are o-phthalaldehyde (OPA), a highly sensitive fluorescent derivatisation reagent for peptide or amino acid detection and quantitation in HPLC.

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Bulk packing, PWXL Top-Off

Supplier: TOSOH Bioscience

Bulk packing, PWXL Top-Off, 1 g wet resin, 10 µm

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Anion exchange bulk packing material

Anion exchange bulk packing material

Supplier: HAMILTON BONADUZ

Polymer bulk packing material for anion exchange applications.

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Adsorbents for column chromatography, Florisil®

Adsorbents for column chromatography, Florisil®

Supplier: MACHEREY-NAGEL

Hard granular magnesia silica gel: MgO (15,5 ±0,5%) SiO2 (84,0 ±0,5%) Na2SO4 (≤1,0%) 60/100 mesh.

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Bulk media for HPLC, NUCLEODUR® C18 HTec

Bulk media for HPLC, NUCLEODUR® C18 HTec

Supplier: MACHEREY-NAGEL

High purity silica phase for preparative HPLC.

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Bulk media for HPLC, NUCLEODUR® C18 ec

Bulk media for HPLC, NUCLEODUR® C18 ec

Supplier: MACHEREY-NAGEL

High purity silica phase for HPLC.

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Reversed phase bulk packing material

Reversed phase bulk packing material

Supplier: HAMILTON BONADUZ

Polymer bulk packing material for reversed phase applications.

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Bulk media for HPLC, POLYGOSIL®

Bulk media for HPLC, POLYGOSIL®

Supplier: MACHEREY-NAGEL

Irregular silica phase for analytical HPLC.

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Bulk media for HPLC, NUCLEODUR® SiOH

Bulk media for HPLC, NUCLEODUR® SiOH

Supplier: MACHEREY-NAGEL

High purity silica phase for HPLC.

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Bulk media for HPLC, POLYGOPREP

Bulk media for HPLC, POLYGOPREP

Supplier: MACHEREY-NAGEL

Irregular silica phase for preparative HPLC.

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Aluminium oxide for preparative chromatography

Supplier: MERCK PRODUCTION CHEMICALS

The aluminium oxide crystal structure comprises octahedrally and tetrahedrally coordinated aluminium coupled by oxygen atoms. The aluminium oxide surface is covered by free hydroxyl groups. There are different acidic and basic centers (Broenstedt acid, Lewis acid and Lewis basic centers) that result in anion and cation exchange properties. Aluminium oxide exhibits a higher pH-stability than silica gel, especially in the alkaline range. Aluminium oxide occurs in various crystal modifications resulting in different pore diameters.

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Micrococcus lysodeikticus, MP Biomedicals

Supplier: MP Biomedicals

Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes and it has also been used in a study to investigate the encapsulation of protein drugs in microparticles.

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Florisil® for preparative chromatography

Supplier: MERCK PRODUCTION CHEMICALS

Composed of magnesium and silicon oxides in the approximate ratio of 85:15. This is a polar phase used for the chromatography of compounds such as steroids and alkaloids. This activity can be controlled by the activation temperature used.

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Cellulose for preparative chromatography

Supplier: MERCK PRODUCTION CHEMICALS

Micro-crystalline cellulose is a hydrophilic polysaccharide packing material preferred for the raw separation of amino acids and related compounds. Cellulose is also used for the gentle purification of bio-molecules. Because of the organic nature of cellulose, it can only be used in pre-swollen state and under gentle or hydrostatic pressure.

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Silica adsorbents for chromatography, EcoChrome™, MP Biomedicals

Supplier: MP Biomedicals

Silica is highly porous and has a number of distinct crystalline forms (polymorphs) in addition to amorphous forms. It is used as a suspending and thickening agent in pharmaceuticals. It is also used in the extraction of DNA and RNA due to its ability to bind to the nucleic acids under the presence of chaotropes.

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Bulk media for ion exchange chromatography columns, PL-SAX

Supplier: VARIAN

The PL-SAX media are well suited for anion exchange HPLC separations of proteins, peptides, and deprotected synthetic oligonucleotides. Denaturing conditions of temperature, organic solvent, and high pH levels are used for the oligonucleotide separations.

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Ion exchangers

Ion exchangers

Supplier: Merck

In general, ion exchangers consist of a cross-linked polymer matrix with a uniform distribution of fixed ionic groups sited throughout the resin structure. These must be balanced by a similar number of ions of the opposite charge, (counter ions) to maintain electrical neutrality. Cation exchangers therefore, exchange and enrich only cations, anion exchangers only anions. In contrast adsorber resins have a non ionic, but depending on the structure, a somewhat polar character, and don't adsorb stoichiometrically both: anions, cations, as well as uncharged compounds.

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Bulk packing material for preparative HPLC columns, SepTech™ C18 reverse phase media

Supplier: VARIAN

SepTech™ C18 bulk media, designed for high performance separations at high capacity. The two pore sizes, 60 Å and 150 Å, have been optimised to give high capacity for small molecules and larger natural molecules and peptides, respectively.

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Bulk media, PLRP-S

Supplier: VARIAN

The PLRP-S reversed phase HPLC media delivers the pure oligonucleotides (oligos) required for genomics applications, including quantitative PCR, microarrays, and gene synthesis assays.

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Bulk media for ion exchange chromatography columns, PL-SCX

Supplier: VARIAN

PL-SCX is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cation exchange functionality. The manufacturing process is controlled to provide optimum density of strong cation-exchange moieties for analysing, separating, and purifying a wide range of biomolecules from small peptides to large proteins.

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