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Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Western blotting detection reagents, ECL western blotting substrate

Western blotting detection reagents, ECL western blotting substrate

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

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Western blotting kit, ECL substrate, Pierce™

Western blotting kit, ECL substrate, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

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Western blot stripping buffer, restore PLUS™

Western blot stripping buffer, restore PLUS™

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

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Western blotting detection reagent, Amersham™ ECL Select™

Western blotting detection reagent, Amersham™ ECL Select™

Supplier: Cytiva

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

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SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Supplier: Thermo Fisher Scientific

The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

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Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Fast western blotting kit, SuperSignal™ west Pico, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

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Western blotting detection reagent, Amersham™ ECL™ Prime

Western blotting detection reagent, Amersham™ ECL™ Prime

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700

VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

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Diluent for western blotting, Swift™ Western Diluent

Diluent for western blotting, Swift™ Western Diluent

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

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Western blots, pre-made, mouse subcellular fraction specific blot

Western blots, pre-made, mouse subcellular fraction specific blot

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

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Immunoblotting kits, MaxTag™

Immunoblotting kits, MaxTag™

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

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Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate

Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate

Supplier: VWR Peqlab

AceGlow™ Chemiluminescence Substrate is for the horseradish peroxidase-mediated detection of proteins and nucleic acids. There are two variants: AceGlow™ Essential for standard blots with a detection sensitivity of 1 picogram and a signal emission for up to 8 hours, and AceGlow™ with highest sensitivity down to 10 femtogram and amplified, long lasting light signal emission over 24 hours.

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Fast western blot kit, SuperSignal™ West Dura, Pierce™

Fast western blot kit, SuperSignal™ West Dura, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.

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Nitrotyrosine BSA for immunoblotting experiments

Nitrotyrosine BSA for immunoblotting experiments

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

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Western blot stripping buffer, Western ReProbe™

Western blot stripping buffer, Western ReProbe™

Supplier: G-Biosciences

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

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Single tissue blots, multiple species

Single tissue blots, multiple species

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

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Western ReProbe™ PLUS

Western ReProbe™ PLUS

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

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Immunodetection reagents, femtoLUCENT™ PLUS

Immunodetection reagents, femtoLUCENT™ PLUS

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

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Cell extract HL60 (non-induced)

Supplier: ENZO LIFE SCIENCES

Whole-cell extract of human HL-60 leukemia cells.

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picoLUCENT™ PLUS-AP kit

picoLUCENT™ PLUS-AP kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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picoLUCENT™ PLUS-HRP Kit

picoLUCENT™ PLUS-HRP Kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Kidney tissue region blot, single species

Kidney tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

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Human cancer cell line blot

Human cancer cell line blot

Supplier: G-Biosciences

Ready to screen human cancer cell line blots.

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Eye tissue region blot, single species

Eye tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.

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Chemiluminescent acridinium esters

Chemiluminescent acridinium esters

Supplier: ENZO LIFE SCIENCES

Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.

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Western blotting detection reagent, Amersham™ ECL™ start

Western blotting detection reagent, Amersham™ ECL™ start

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

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Normal cell line blots, human

Normal cell line blots, human

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

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CYTAG™ CGH labelling kits

CYTAG™ CGH labelling kits

Supplier: ENZO LIFE SCIENCES

The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.

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IHC chromogen substrate (AEC, stable), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.

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Multiple tissue blot, single species

Multiple tissue blot, single species

Supplier: G-Biosciences

These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.

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