Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Western blotting detection reagents, ECL western blotting substrate
Supplier: Thermo Fisher Scientific
ECL™ Western blotting substrate is an entry-level Western blotting substrate.
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Western blotting kit, ECL substrate, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
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Western blot stripping buffer, restore PLUS™
Supplier: Thermo Fisher Scientific
Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.
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Western blotting detection reagent, Amersham™ ECL Select™
Supplier: Cytiva
Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
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SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto
Supplier: Thermo Fisher Scientific
The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.
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Fast western blotting kit, SuperSignal™ west Pico, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.
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Western blotting detection reagent, Amersham™ ECL™ Prime
Supplier: Cytiva
Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge
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Diluent for western blotting, Swift™ Western Diluent
Supplier: G-Biosciences
The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.
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Western blots, pre-made, mouse subcellular fraction specific blot
Supplier: G-Biosciences
The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
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Immunoblotting kits, MaxTag™
Supplier: Rockland Immunochemicals
These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.
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Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate
Supplier: VWR Peqlab
AceGlow™ Chemiluminescence Substrate is for the horseradish peroxidase-mediated detection of proteins and nucleic acids. There are two variants: AceGlow™ Essential for standard blots with a detection sensitivity of 1 picogram and a signal emission for up to 8 hours, and AceGlow™ with highest sensitivity down to 10 femtogram and amplified, long lasting light signal emission over 24 hours.
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Fast western blot kit, SuperSignal™ West Dura, Pierce™
Supplier: Thermo Fisher Scientific
The Fast Western Blot Kits, SuperSignal™ West Dura provide reagents and protocol for reliable and rapid, 1 hour chemiluminescent Western blotting at femtogram levels of sensitivity. These complete Fast Western Blot Kits requires minimal hands on time and yield results comparable to classical Western blotting with SuperSignal™ West Dura Chemiluminescent Substrate. This substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with mouse or rabbit primary antibody.
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Nitrotyrosine BSA for immunoblotting experiments
Supplier: Cayman Chemical
This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.
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Western blot stripping buffer, Western ReProbe™
Supplier: G-Biosciences
Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.
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Single tissue blots, multiple species
Supplier: G-Biosciences
Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.
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Western ReProbe™ PLUS
Supplier: G-Biosciences
Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.
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Immunodetection reagents, femtoLUCENT™ PLUS
Supplier: G-Biosciences
The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.
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Cell extract HL60 (non-induced)
Supplier: ENZO LIFE SCIENCES
Whole-cell extract of human HL-60 leukemia cells.
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picoLUCENT™ PLUS-AP kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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picoLUCENT™ PLUS-HRP Kit
Supplier: G-Biosciences
The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.
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Kidney tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.
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Human cancer cell line blot
Supplier: G-Biosciences
Ready to screen human cancer cell line blots.
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Eye tissue region blot, single species
Supplier: G-Biosciences
These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.
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Chemiluminescent acridinium esters
Supplier: ENZO LIFE SCIENCES
Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.
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Western blotting detection reagent, Amersham™ ECL™ start
Supplier: Cytiva
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
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Normal cell line blots, human
Supplier: G-Biosciences
Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.
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CYTAG™ CGH labelling kits
Supplier: ENZO LIFE SCIENCES
The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.
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IHC chromogen substrate (AEC, stable), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.
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Multiple tissue blot, single species
Supplier: G-Biosciences
These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.