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Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
DNA ladder, O'RangeRuler™
Supplier: Thermo Fisher Scientific
O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.
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Lambda DNA/HindIII Marker
Supplier: Thermo Fisher Scientific
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
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Lambda DNA phage, Hind III digest
Supplier: Rockland Immunochemicals
Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.
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DNA marker, pBR322 DNA/BsuRI (HaeIII) marker
Supplier: Thermo Fisher Scientific
pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.
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DNA ladder 250 to 10000 bp, AMPIGENE®
Supplier: Enzo Life Sciences
AMPIGENE® DNA ladder contains DNA fragments ranging from 250 to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 1000 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
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Unstained Protein Ladder (10 - 200 kDa), Abcam
Supplier: Abcam
Unstained Protein Ladder ab234618 contains 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.
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100 bp DNA Ladder, Abcam
Supplier: Abcam
This product is manufactured by BioVision, an Abcam company and was previously called M1158, 100 bp DNA ladder.
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Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa), Abcam
Supplier: Abcam
Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
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DNA ladder 50 to 1500 bp, AMPIGENE®
Supplier: Enzo Life Sciences
AMPIGENE® DNA ladder contains DNA fragments ranging from 50 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 200 bp, 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
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DNA ladders
Supplier: Biotium
Ready to use DNA ladders are supplied in 1X loading buffer at an optimal concentration for loading GelRed™ and GelGreen™ precast gels.
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Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa), Abcam
Supplier: Abcam
10-180 kDa prestained protein ladder is a three-color protein standard with 10 pre-stained proteins. - Suitable for western blot (WB), SDS-PAGE - Designed for monitoring protein separation - Supplied in gel loading buffer and is ready to use
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Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa), Abcam
Supplier: Abcam
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa.
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Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa), Abcam
Supplier: Abcam
5-245 kDa prestained protein ladder is a three-color protein standard with 13 prestained proteins. - Suitable for western blot (WB), SDS-PAGE - Designed for monitoring protein separation - Supplied in gel loading buffer and is ready to use
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Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa), Abcam
Supplier: Abcam
High molecular weight prestained protein ladder 10-245 kDa is a three-color protein standard with 12 prestained proteins. - Cited in over 15 publications - Suitable for western blot (WB), SDS-PAGE - Designed for monitoring protein separation - Supplied in gel loading buffer and is ready to use
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DNA Ladder 100 to 10000 bp, AMPIGENE®
Supplier: Enzo Life Sciences
AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp, 1500 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
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DNA ladder 100 to 1500 bp, AMPIGENE®
Supplier: Enzo Life Sciences
AMPIGENE® DNA ladder contains DNA fragments ranging from 100 bp to 1500 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 500 bp and 1500 bpto serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
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Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa), Abcam
Supplier: Abcam
Prestained Protein Ladder AB313996 is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
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DNA ladders, GeneRuler™ and O'GeneRuler™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.
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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits
Supplier: VWR Chemicals
VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.
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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700
Supplier: VWR Chemicals
VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge
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Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate
Supplier: VWR Collection
AceGlow™ Chemiluminescence Substrate is for the horseradish peroxidase-mediated detection of proteins and nucleic acids. There are two variants: AceGlow™ Essential for standard blots with a detection sensitivity of 1 picogram and a signal emission for up to 8 hours, and AceGlow™ with highest sensitivity down to 10 femtogram and amplified, long lasting light signal emission over 24 hours.
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ECL Substrate Kit (Very High Sensitivity), Abcam
Supplier: Abcam
Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.
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Optiblot SDS-PAGE Sample Preparation Kit, Abcam
Supplier: Abcam
The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
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Normal cell line blots, human
Supplier: G-Biosciences
Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.
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CYTAG™ CGH labelling kits
Supplier: Enzo Life Sciences
The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.
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Antibody Diluent 4BR, Abcam
Supplier: Abcam
Antibody Diluent 4BR (ab309955) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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IHC chromogen substrate (AEC, stable), HIGHDEF®
Supplier: Enzo Life Sciences
HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.
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Ethidium bromide Greenbag™ disposal kit, MP Biomedicals
Supplier: MP Biomedicals
The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.
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Diluent for western blotting, Swift™ Western Diluent
Supplier: G-Biosciences
The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.
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Antibody Diluent, Abcam
Supplier: Abcam
Antibody diluent (pH 7 - 7.8) is used to dilute primary antibodies to their working concentrations for use in immunohistochemistry (IHC). - Cited in over 15 publications - Reduces background and non-specific binding - Extends antibody stability up to 24 months at 2-8°C - Tris-buffered with stabilizing protein, wetting agent, antimicrobial agent, and green dye