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Supplier: G-Biosciences

SilverOUT removes interfering silver ions from gel pieces.

Supplier: G-Biosciences

The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

Supplier: G-Biosciences

Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

Supplier: G-Biosciences

InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

Supplier: G-Biosciences

Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

Supplier: Enzo Life Sciences

Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

Supplier: G-Biosciences

Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Supplier: G-Biosciences

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

Supplier: Thermo Fisher Scientific

Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

Supplier: G-Biosciences

Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

Supplier: Thermo Fisher Scientific

Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

Supplier: Thermo Fisher Scientific

Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

Supplier: Abcam

Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. - Cited in over 20 publications - Colour-coded pH indicator for accuracy - Superior pH stability at high temperatures - Non-toxic, odorless, and azide-free - Optimized for FFPE tissue antigen retrieval

Supplier: G-Biosciences

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Supplier: G-Biosciences

DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Supplier: Abcam

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.