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Proteinové čistící reagence

Proteinové čistící reagence

Reagencie pro purifikaci proteinů se používají k izolaci jednoho nebo více proteinů z komplexní směsi, obvykle buněk, tkání nebo celých organismů. Tento proces je zásadní pro charakterizaci funkce a struktury a také pro identifikaci interakcí cílového proteinu. V průběhu mnoha kroků pomáhají reagencie pro purifikaci proteinů při lýze buněk, oddělení rozpustných proteinových složek od buněčných zbytků a nakonec při oddělení cílového proteinu od nečistot souvisejících s produktem a procesem.

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HRP Conjugate Stabilizer - LifeXtend, Abcam

Supplier: Abcam

HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature.

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Destičky pro kapalinovou extrakci, J.T.Baker® Bakerbond®

Destičky pro kapalinovou extrakci, J.T.Baker® Bakerbond®

Supplier: Avantor

Destičky J.T.Baker® BAKERBOND® pro kapalinovou extrakci (SLE) jsou ideální a rychlejší alternativou k tradičním metodám extrakce kapalina-kapalina a umožňují vynikající výtěžky analytů z biologických matric.

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Destičky k precipitaci proteinu J.T.Baker® Bakerbond®

Destičky k precipitaci proteinu J.T.Baker® Bakerbond®

Supplier: Avantor

Destičky k precipitaci proteinu J.T.Baker® Bakerbond® umožňují rychlé odstranění proteinů z biologických vzorků.

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Proteomic protein controls

Supplier: G-Biosciences

Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, E.coli, yeast, plant or as a control set that contains one 2 mg vial of each.

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Immobilised D-galactose for lectin purification

Immobilised D-galactose for lectin purification

Supplier: G-Biosciences

Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.

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Diluent M Concentrate (10x)

Diluent M Concentrate (10x)

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Jacalin for IgA purification

Jacalin for IgA purification

Supplier: G-Biosciences

Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (Artocarpus integrifolia) seeds.

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TMB substrate 1X

TMB substrate 1X

Supplier: Abcam

TMB substrate recommended for use with the Matched Antibody Pair kits for sandwich ELISA.

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Sample Diluent NBS

Sample Diluent NBS

Supplier: Abcam

Sample Diluent NBS (ab221827) is part of the reagents, controls and accessories range. High-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates are available.

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Concanavalin A agarose

Concanavalin A agarose

Supplier: G-Biosciences

Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

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HRP Stability Conjugate Reagent

HRP Stability Conjugate Reagent

Supplier: Abcam

Abcam's HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution.

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Sample Diluent NBP

Sample Diluent NBP

Supplier: Abcam

Sample Diluent NBP is part of the reagents, controls and accessories range.

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Streptavidin-HRP Solution

Streptavidin-HRP Solution

Supplier: Abcam

Streptavidin-HRP Solution (ab210901) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Pep-Extract™

Supplier: G-Biosciences

Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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Recombinant protein L, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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