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Supplier: Thermo Fisher Scientific

dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.

Supplier: TriLink BioTechnologies

Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.

Supplier: Thermo Fisher Scientific

This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: MP Biomedicals

The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT Bioquest

3'-O-Azidomethyl-dCTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

Supplier: Thermo Fisher Scientific

The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Supplier: AAT Bioquest

2'-Deoxyguanosine-5'-Triphosphate (dGTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Supplier: AAT Bioquest

ReadiUse™ ddNTP Terminator Mix consists of 4 equimolar 2',3'-ddNTPs, including ddATP, ddGTP, ddTTP and ddCTP.

Supplier: AAT Bioquest

iFluor® 560-dUTP is a iFluor 560-modified nucleotide that can be used in various molecular biology applications, such as labelling and detection of DNA molecules.

Supplier: AAT Bioquest

Fluorescein-12-dGTP might be incorporated into a DNA sequence as its natural counterpart dGTP to incorporate a fluorescein tag in a DNA molecule.

Supplier: TriLink BioTechnologies

CleanCap® Reagent AG is TriLink’s patented co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency.

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5' capped mRNA resulting in a Cap 1 structure with >95% capping efficiency.

Supplier: Thermo Fisher Scientific

dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Supplier: AAT Bioquest

3'-O-Azidomethyl-dGTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

Supplier: AAT Bioquest

Fluorescein-12-dATP might be incorporated into a DNA sequence as its natural counterpart dATP to incorporate a fluorescein tag in a DNA molecule.

Supplier: AAT Bioquest

This iFluor® 488-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™ Alexa Fluor 488-dUTP.

Supplier: AAT Bioquest

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labelling or PCR.

Supplier: AAT Bioquest

Sanger method is one of the most reliable and earliest DNA sequencing methods.

Supplier: AAT Bioquest

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Supplier: AAT Bioquest

This iFluor® 555-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™ Alexa Fluor 555-dUTP.

Supplier: AAT Bioquest

3'-O-Azidomethyl-dATP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

Supplier: TriLink BioTechnologies

T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by in vitro transcription.

Supplier: AAT Bioquest

This iFluor 488-dUTP conjugate is used as a green fluorescence colour with SpectrumGreen™ filter set (SpectrumGreen™ is the trademark of Vysis).

Supplier: AAT Bioquest

This Cy5-dUTP conjugate can be used as a red fluorescence colour with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis).

Supplier: AAT Bioquest

2'-Deoxycytidine-5'-Triphosphate (dCTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.

Supplier: AAT Bioquest

This fluorescein-12-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).